Suplementação dietária de cobre no desempenho produtivo da tilápia-do-Nilo e resposta hemática sob estresse térmico
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Data
2013-06-05
Autores
Damasceno, Flávia Mota [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O mineral cobre é considerado essencial para peixes por ser componente de diversas enzimas e participar em importantes reações orgânicas. Entretanto, altos níveis deste mineral podem prejudicar a homeostase e causar sérios danos nos tecidos. Os efeitos de dietas contendo níveis crescentes de cobre no desempenho, respostas hematológicas, atividade da ceruloplasmina, histologia do fígado e resistência da tilápia-do-Nilo ao estresse térmico foram analisados, com o objetivo de avaliar as respostas fisiológicas sob condições de estresse e desempenho produtivo frente aos níveis de suplementação dietária de cobre. Duzentos e quarenta peixes (peso inicial 8,05 ± 0,45 g) foram, aleatoriamente, distribuídos em trinta aquários (250 L) e arraçoados com dietas práticas suplementadas com cinco níveis de Cu2SO4.5H2O (4,0; 6,0; 8,0; 1000,0 e 1500 mg Cu.kg-1) e uma dieta sem suplementação, pelo período experimental de cento e vinte dias. Ao fim do período experimental foi determinado o desempenho (ganho de peso, consumo de ração, conversão alimentar, taxa de crescimento específico, taxa de retenção proteica e sobrevivência), e foram feitas análises histológicas dos fígados. Os parâmetros hematológicos e bioquímico (eritrócitos, hemoglobina, hematócrito, volume corpuscular médio, concentração de hemoglobina corpuscular média, proteína plasmática total, albumina, globulina, relação albumina:globulina e atividade da ceruloplasmina) foram avaliados após o período experimental, e em seguida os peixes foram submetidos ao estresse térmico (32°C) por três dias e, posteriormente, determinados os mesmos parâmetros hematológicos e bioquímicos. Os dados de desempenho foram submetidos à técnica de análise de variância (P<0,05), e os dados hematológicos foram submetidos ao teste não-paramétrico de Kruskal-Wallis; e complementados com o teste de comparações de Mann-Whitne...
Copper is considered essential for fish for being component of several enzymes and to participate in important reactions of organism. However, high levels of this mineral can impair the homeostasis and cause serious damages on tissues. The effects of feeding diets containing graded levels of copper on growth performance, haematological response, activity of ceruloplasmin and histology of liver, and the resistance to a high temperature stress of Nile tilapia were analyzed, in order to evaluate the physiological responses under stress conditions and growth performance front the increasing levels of dietary supplementation of copper. Two hundred and forty juveniles of Nile tilapia (initial weight 8.05 g/fish) were randomly distribute in 30 250 L-aquaria and fed with five practical diets containing levels of Cu2SO4.5H2O (4.0; 6.0; 8.0; 1000 and 1500 mg Cu.kg-1 diet) and a diet without supplementation, for 120 days. At the end of experimental period growth performance (weigh gain, feed intake, feed conversion rate, specific growth rate, protein retention rate and survival) were calculated, and the liver of five fishes/treatment were collected to histological analyses. The haematological and biochemical parameters (red blood count, haematocrit, haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentration, total plasmatic protein, albumin, globulin, albumin/globulin ratio and ceruloplasmin activity) were evaluated after the experimental period, and then the fishes were subjected to heat stress (32°C) for three days, after the same haematological parameters were evaluated. The data of growth performance were submitted to analysis of variance (P<0,05). Haematological parameters were submitted to non-parametric test of Kruskal-Wallis; and complemented with comparasion test of Mann-Whitney (P<0,05). The toxicity of dietary copper impaired significantly the growth performance of groups of fish ...
Copper is considered essential for fish for being component of several enzymes and to participate in important reactions of organism. However, high levels of this mineral can impair the homeostasis and cause serious damages on tissues. The effects of feeding diets containing graded levels of copper on growth performance, haematological response, activity of ceruloplasmin and histology of liver, and the resistance to a high temperature stress of Nile tilapia were analyzed, in order to evaluate the physiological responses under stress conditions and growth performance front the increasing levels of dietary supplementation of copper. Two hundred and forty juveniles of Nile tilapia (initial weight 8.05 g/fish) were randomly distribute in 30 250 L-aquaria and fed with five practical diets containing levels of Cu2SO4.5H2O (4.0; 6.0; 8.0; 1000 and 1500 mg Cu.kg-1 diet) and a diet without supplementation, for 120 days. At the end of experimental period growth performance (weigh gain, feed intake, feed conversion rate, specific growth rate, protein retention rate and survival) were calculated, and the liver of five fishes/treatment were collected to histological analyses. The haematological and biochemical parameters (red blood count, haematocrit, haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin concentration, total plasmatic protein, albumin, globulin, albumin/globulin ratio and ceruloplasmin activity) were evaluated after the experimental period, and then the fishes were subjected to heat stress (32°C) for three days, after the same haematological parameters were evaluated. The data of growth performance were submitted to analysis of variance (P<0,05). Haematological parameters were submitted to non-parametric test of Kruskal-Wallis; and complemented with comparasion test of Mann-Whitney (P<0,05). The toxicity of dietary copper impaired significantly the growth performance of groups of fish ...
Descrição
Palavras-chave
Tilapia (Peixe), Cobre na nutrição animal, Figado - Histopatologia, Peixe - Alimentação, Fishes Food
Como citar
DAMASCENO, Flávia Mota. Suplementação dietária de cobre no desempenho produtivo da tilápia-do-Nilo e resposta hemática sob estresse térmico. 2013. 63 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária e Zootecnia, 2013.