Avaliação da expressão de genes-alvo do receptor ativado por proliferadores do peroxissoma alfa (PPaRα), em indivíduos obesos e não-obesos, em situações de jejum
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Data
2011-08-26
Autores
Ingrid Felicidade [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Em diversas partes do mundo, a prevalência da obesidade tem aumentado rapidamente. A Organização Mundial da Saúde estima que, em 2015, haverá 700 milhões de pessoas obesas (índice de massa corporal ≥30kg/m2) em todo o mundo. No Brasil, aproximadamente, 50% da população acima dos 20 anos apresentam sobrepeso (índice de massa corporal ≥25kg/m2) e 16% já demonstram quadro de obesidade. A obesidade é um dos fatores desencadeadores de diversas doenças crônicas, assim, é importante identificar características que predispõem indivíduos à obesidade, ou condições negativas que ocorrem durante o desenvolvimento da obesidade, as quais alteram o metabolismo e o estado de saúde de maneira negativa. O objetivo do presente estudo foi avaliar diferenças na expressão de genes-alvo do PPARα em células mononucleares periféricas do sangue (PBMCs) e na concentração plasmática de ácidos graxos livres no sangue, entre indivíduos obesos e não-obesos, após 24 horas de jejum. Este estudo foi o primeiro realizado na população brasileira. Em ambos os grupos (obeso e não-obeso), a concentração plasmática de ácidos graxos livres (AGL) elevou-se de forma significante após as 24horas de jejum, em especial, no grupo não-obeso, onde a média da concentração elevou-se 3 vezes se comparada com a média da concentração no tempo 0h. A média da concentração plasmática basal (0h) de AGL revelou-se 2 vezes maior no grupo obeso quando comparada com a média do grupo não-obeso. No entanto essa diferença não foi observada após as 24h de jejum. Os genes estudados participam diretamente na β-oxidação mitocondrial hepática e musculoesquelética e são ativados por PPARα. Após o jejum, todos os genes estudados evidenciaram um incremento na expressão (fold change) quando comparados com o...
The prevalence of obesity has increased rapidly in many parts of the world. The World Health Organization estimates that in 2015, there should be 700 million obese people (body mass index ≥ 30kg / m²) around the world. In Brazil, approximately 50% of the population aged 20 years and older are overweight (body mass index ≥ 25kg / m²) and 16% already meet the criterion for obesity. Obesity is considered a trigger factor for several chronic diseases, so it is important to identify traits that predispose individuals to obesity or negative conditions that occur during the development of obesity, affecting metabolism and health negatively. The aim of this study was to evaluate differences in the expression of PPAR-alpha target genes in peripheral blood mononuclear cells (PBMCs) and in plasma free fatty acid concentration in the blood of obese and non-obese subjects, after 24 hours of fasting. This was the first study conducted in the Brazilian population. In both groups (obese and non-obese), plasma free fatty acid (FFA) concentration increased significantly after 24 hours of fasting, especially in the non-obese group, in which the mean concentration rose 3 times compared with the mean concentration at time 0h. The baseline (0h) mean of plasma FFA concentration was 2 times higher in the obese group compared with the mean from the non-obese group. This difference, however, was not observed after 24h of fasting. The studied genes participate directly in hepatic and musculoskeletal mitochondrial beta-oxidation and are activated by PPAR-alpha. After fasting, all the studied genes had an increase in fold change compared with time 0h, in both groups. By identifying the preferential beta-oxidation during fasting, the CPT1A gene presented an increase in fold change compared with the CPT1B gene, indicating thus that beta-oxidation... (Complete abstract click electronic access below)
The prevalence of obesity has increased rapidly in many parts of the world. The World Health Organization estimates that in 2015, there should be 700 million obese people (body mass index ≥ 30kg / m²) around the world. In Brazil, approximately 50% of the population aged 20 years and older are overweight (body mass index ≥ 25kg / m²) and 16% already meet the criterion for obesity. Obesity is considered a trigger factor for several chronic diseases, so it is important to identify traits that predispose individuals to obesity or negative conditions that occur during the development of obesity, affecting metabolism and health negatively. The aim of this study was to evaluate differences in the expression of PPAR-alpha target genes in peripheral blood mononuclear cells (PBMCs) and in plasma free fatty acid concentration in the blood of obese and non-obese subjects, after 24 hours of fasting. This was the first study conducted in the Brazilian population. In both groups (obese and non-obese), plasma free fatty acid (FFA) concentration increased significantly after 24 hours of fasting, especially in the non-obese group, in which the mean concentration rose 3 times compared with the mean concentration at time 0h. The baseline (0h) mean of plasma FFA concentration was 2 times higher in the obese group compared with the mean from the non-obese group. This difference, however, was not observed after 24h of fasting. The studied genes participate directly in hepatic and musculoskeletal mitochondrial beta-oxidation and are activated by PPAR-alpha. After fasting, all the studied genes had an increase in fold change compared with time 0h, in both groups. By identifying the preferential beta-oxidation during fasting, the CPT1A gene presented an increase in fold change compared with the CPT1B gene, indicating thus that beta-oxidation... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Biologia molecular, Obesidade, Jejum, Acidos graxos, Free fatty acids, Obesity
Como citar
Avaliação da expressão de genes-alvo do receptor ativado por proliferadores do peroxissoma alfa (PPaRα), em indivíduos obesos e não-obesos, em situações de jejum. 2011. 58 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina de Botucatu, 2011.