Influência do plasma rico em plaquetas no reparo ósseo de defeitos de tamanho crítico em calvárias de ratos: estudo histológico e histométrico
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Data
2005-11-29
Autores
Messora, Michel Reis [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Objetivo: O propósito deste estudo foi avaliar, histologicamente, a influência do Plasma Rico em Plaquetas (PRP) no reparo ósseo de defeitos de tamanho crítico (DTC) criados cirurgicamente em calvárias de ratos. Material e Método: 32 ratos foram divididos em 2 grupos: C (controle) e PRP (Plasma Rico em Plaquetas). Um DTC de 8 mm de diâmetro foi criado na calvária de cada animal. No Grupo C, o defeito foi preenchido somente com coágulo sangüíneo. No Grupo PRP, o defeito foi preenchido com Plasma Rico em Plaquetas. Cada grupo foi subdividido para eutanásia em 4 ou 12 semanas pós-operatórias (n=8). Foram realizadas análises histológica e histométrica. A quantidade de osso neoformado foi calculada como uma proporção da área total do defeito original. Esses valores foram transformados em arcoseno para a análise estatística (ANOVA, Tukey, p < 0.05). Resultados: Nenhum defeito reparou completamente com tecido ósseo. O Grupo PRP apresentou significativamente mais neoformação óssea que o Grupo C, tanto em 4 semanas (17,68% e 7,20%, respectivamente) como em 12 semanas (24,69% e 11,65%, respectivamente) pós-operatórias. Conclusão: Dentro dos limites deste estudo, pode-se concluir que o PRP aumentou significativamente o reparo ósseo de defeitos de tamanho crítico em calvárias de ratos.
The purpose of this study was to histologically analyze the influence of Platelet Rich Plasma (PRP) on bone healing in surgically created critical-size-defects (CSD) in rat calvaria. 32 rats were divided into 2 groups: C (control) and PRP (Platelet Rich Plasma). An 8 mm diameter CSD was created in the calvarium of each animal. In Group C the defect was filled by blood clot only. In Group PRP it was filled with Platelet Rich Plasma. Both groups were divided into subgroups (n=8) and euthanized at either 4 or 12 weeks post-operative. Histometric, using image analysis software, and histologic analyses were performed. Amount of new bone was calculated as percentage of total area of original defect. Percentage data were transformed into arccosine for statistical analysis (ANOVA, Tukey, p < 0.05). No defect completely regenerated with bone. Group PRP had a statistically greater amount of bone formation than Group C at both 4 (17.68% and 7.20%, respectively) and 12 weeks (24.69% and 11.65%, respectively) post-operative. Within the limits of this study, it can be concluded that PRP significantly enhanced bone healing in critical-size-defects in rat calvaria.
The purpose of this study was to histologically analyze the influence of Platelet Rich Plasma (PRP) on bone healing in surgically created critical-size-defects (CSD) in rat calvaria. 32 rats were divided into 2 groups: C (control) and PRP (Platelet Rich Plasma). An 8 mm diameter CSD was created in the calvarium of each animal. In Group C the defect was filled by blood clot only. In Group PRP it was filled with Platelet Rich Plasma. Both groups were divided into subgroups (n=8) and euthanized at either 4 or 12 weeks post-operative. Histometric, using image analysis software, and histologic analyses were performed. Amount of new bone was calculated as percentage of total area of original defect. Percentage data were transformed into arccosine for statistical analysis (ANOVA, Tukey, p < 0.05). No defect completely regenerated with bone. Group PRP had a statistically greater amount of bone formation than Group C at both 4 (17.68% and 7.20%, respectively) and 12 weeks (24.69% and 11.65%, respectively) post-operative. Within the limits of this study, it can be concluded that PRP significantly enhanced bone healing in critical-size-defects in rat calvaria.
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Palavras-chave
Ossos - Regeneração, Substancias de crescimento, Plaquetas (Sangue), Regeneração óssea, Bone regeneration, Growth substances, Blood platelets
Como citar
MESSORA, Michel Reis. Influência do plasma rico em plaquetas no reparo ósseo de defeitos de tamanho crítico em calvárias de ratos: estudo histológico e histométrico. 2005. 144 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2005.