A Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samples

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dc.contributor.authorKhan, Sabir [UNESP]
dc.contributor.authorWong, Ademar [UNESP]
dc.contributor.authorRychlik, Michael
dc.contributor.authorSotomayor, María del Pilar Taboada [UNESP]
dc.contributor.institutionFederal Rural University of the Semi-Arid
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionTechnical University of Munich
dc.contributor.institutionToxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM)
dc.date.accessioned2023-07-29T16:07:50Z
dc.date.available2023-07-29T16:07:50Z
dc.date.issued2022-11-01
dc.description.abstractThe present study reports the development and application of a novel, sensitive, and selective voltammetric sensor for the quantitation of folate or vitamin B9 in foodstuffs. The sensor was made from magnetic molecularly imprinted polymers (MMIPs), which were synthesized by the core–shell method using magnetite nanoparticles obtained by the polyol method. The MMIP-based sensor was used for the selective and specific detection of folate in different food samples. The MMIP material was constructed using magnetic water-dispersible nanomaterial, which was prepared by immersing iron (III) acetylacetonate in tri-ethylene-glycol (TEG) solvent. The magnetic water-dispersible nanomaterial was then subjected to polymerization using allyl alcohol as a functional monomer, ethylene-glycol-dimethacrylate (EGDMA) as a cross-linking agent, and 2,2-Azobisisobutyronitrile (AIBN) as a radical initiator. The proposed magnetic materials were characterized by Brunauer–Emmett–Teller (BET), field emission gun scanning electron microscopy (FEG-SEM), thermogravimetric analysis (TGA), and vibrating sample magnetometer (VSM) analysis. The quantification of folate was performed by square wave voltammetry under optimized conditions using 15 mg of MMIPs and 85 mg of carbon paste. The modified electrode presented a linear dynamic range (LDR) of 2.0–12 µmol L−1 and a limit of detection (LOD) of 1.0 × 10−7 mol L−1 in 0.1 mol L−1 acetate buffer solution (pH 4.0). The proposed sensor was successfully applied for folate detection in different food samples, where recovery percentages ranging from 93 to 103% were obtained. Finally, the results obtained from the analysis of selectivity showed that the modified biomimetic sensor is highly efficient for folate determination in real food samples. Adsorption tests were used to evaluate and compare the efficiency of the MMIPs and magnetic non-molecularly imprinted polymer (MNIPs)—used as control material, through the application of HPLC as a standard method.en
dc.description.affiliationDepartment of Natural Sciences Mathematics and Statistics Federal Rural University of the Semi-Arid
dc.description.affiliationInstitute of Chemistry São Paulo State University (UNESP)
dc.description.affiliationAnalytical Food Chemistry School of Life Sciences Technical University of Munich, Maximus-von-Imhof-Forum 2
dc.description.affiliationNational Institute of Alternative Technologies for Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM)
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: #151717/2018-4
dc.description.sponsorshipIdFAPESP: #2014/50945-4
dc.description.sponsorshipIdFAPESP: #2019/00677-7
dc.description.sponsorshipIdCNPq: #301728/2019-4
dc.description.sponsorshipIdCNPq: #408050/2018 7
dc.description.sponsorshipIdCNPq: #465571/2014-0
dc.identifierhttp://dx.doi.org/10.3390/chemosensors10110473
dc.identifier.citationChemosensors, v. 10, n. 11, 2022.
dc.identifier.doi10.3390/chemosensors10110473
dc.identifier.issn2227-9040
dc.identifier.scopus2-s2.0-85149484287
dc.identifier.urihttp://hdl.handle.net/11449/249738
dc.language.isoeng
dc.relation.ispartofChemosensors
dc.sourceScopus
dc.subjectelectrochemical sensor
dc.subjectfolate
dc.subjectMMIPs
dc.subjectreal samples
dc.titleA Novel Synthesis of a Magnetic Porous Imprinted Polymer by Polyol Method Coupled with Electrochemical Biomimetic Sensor for the Detection of Folate in Food Samplesen
dc.typeArtigo
unesp.author.orcid0000-0003-4557-238X[1]
unesp.author.orcid0000-0002-5826-6288[3]
unesp.author.orcid0000-0002-6173-7888[4]

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Artigos - Química Analítica - IQ