Xenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspring

dc.contributor.authorChiaratti, Marcos Roberto
dc.contributor.authorFerreira, Christina Ramires [UNESP]
dc.contributor.authorMeirelles, Flavio Vieira
dc.contributor.authorMeo, Simone Cristina [UNESP]
dc.contributor.authorPerecin, Felipe
dc.contributor.authorSmith, Lawrence Charles
dc.contributor.authorFerraz, Marcio Leao
dc.contributor.authorde Sa Filho, Manoel Francisco
dc.contributor.authorGimenes, Lindsay Unno
dc.contributor.authorBaruselli, Pietro Sampaio
dc.contributor.authorGasparrini, Bianca
dc.contributor.authorGarcia, Joaquim Mansano [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniv Montreal
dc.contributor.institutionVida Reprodut Consultoria
dc.contributor.institutionUniv Naples Federico 2
dc.date.accessioned2014-05-20T13:15:23Z
dc.date.available2014-05-20T13:15:23Z
dc.date.issued2010-06-01
dc.description.abstractNuclear-mitochondrial incompatibilities may be responsible for the development failure reported in embryos and fetuses produced by interspecies somatic cell nuclear transfer (iSCNT). Herein we performed xenooplasmic transfer (XOT) by introducing 10 to 15% of buffalo ooplasm into bovine zygotes to assess its effect on the persistence of buffalo mitochondrial DNA (mtDNA). Blastocyst rates were not compromised by XOT in comparison to both in vitro fertilized embryos and embryos produced by transfer of bovine ooplasm into bovine zygotes. Moreover, offspring were born after transfer of XOT embryos to recipient cows. Buffalo mtDNA introduced in zygotes was still present at the blastocyst stage (8.3 vs. 9.3%, p = 0.11), indicating unaltered heteroplasmy during early development. Nonetheless, no vestige of buffalo mtDNA was found in offspring, indicating a drift to homoplasmy during later stages of development. In conclusion, we show that the buffalo mtDNA introduced by XOT into a bovine zygote do not compromise embryo development. on the other hand, buffalo mtDNA was not inherited by offspring indicating a possible failure in the process of interspecies mtDNA replication.en
dc.description.affiliationUSP, Dept Ciencias Basicas, FZEA, Pirassununga, SP, Brazil
dc.description.affiliationUNESP, FCAV, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, Brazil
dc.description.affiliationEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuaria Sudeste, Sao Carlos, SP, Brazil
dc.description.affiliationUniv Montreal, CRRA, St Hyacinthe, PQ, Canada
dc.description.affiliationVida Reprodut Consultoria, Cravinhos, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Anim Reprod, São Paulo, Brazil
dc.description.affiliationUniv Naples Federico 2, DISCIZIA, Naples, Italy
dc.description.affiliationUnespUNESP, FCAV, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 02/05054-7
dc.description.sponsorshipIdFAPESP: 04/01841-0
dc.description.sponsorshipIdFAPESP: 06/59074-0
dc.format.extent231-236
dc.identifierhttp://dx.doi.org/10.1089/cell.2009.0076
dc.identifier.citationCellular Reprogramming. New Rochelle: Mary Ann Liebert Inc., v. 12, n. 3, p. 231-236, 2010.
dc.identifier.doi10.1089/cell.2009.0076
dc.identifier.fileWOS000279402900001.pdf
dc.identifier.issn2152-4971
dc.identifier.lattes9472065929531215
dc.identifier.lattes2251116139872527
dc.identifier.urihttp://hdl.handle.net/11449/2546
dc.identifier.wosWOS:000279402900001
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofCellular Reprogramming
dc.relation.ispartofjcr1.430
dc.relation.ispartofsjr0,549
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleXenooplasmic Transfer between Buffalo and Bovine Enables Development of Homoplasmic Offspringen
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/archpolicy/journal-of-womens-health/42/
dcterms.rightsHolderMary Ann Liebert Inc.
unesp.author.lattes9472065929531215
unesp.author.lattes2251116139872527
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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