Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major

dc.contributor.authorCosta Tonoli, Celisa Caldana
dc.contributor.authorVieira, Plinio Salmazo
dc.contributor.authorWard, Richard John
dc.contributor.authorArni, Raghuvir Krishnaswamy [UNESP]
dc.contributor.authorCavalcante de Oliveira, Arthur Henrique
dc.contributor.authorMurakami, Mario Tyago
dc.contributor.institutionBrazilian Assoc Synchrotron Light Technol
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:02:41Z
dc.date.available2014-05-20T14:02:41Z
dc.date.issued2009-11-01
dc.description.abstractNucleoside diphosphate kinases (NDKs; EC 2.7.4.6) play an essential role in the synthesis of nucleotides from intermediates in the salvage pathway in all parasitic trypanosomatids and their structural studies will be instrumental in shedding light on the biochemical machinery involved in the parasite life cycle and host-parasite interactions. In this work, NDKb from Leishmania major was overexpressed in Escherichia coli, purified to homogeneity and crystallized using the sitting-drop vapour-diffusion method. The NDK crystal diffracted to 2.2 angstrom resolution and belonged to the trigonal crystal system, with unit-cell parameters a = 114.2, c = 93.9 angstrom. Translation-function calculations yielded an unambiguous solution in the enantiomorphic space group P3(2)21.en
dc.description.affiliationBrazilian Assoc Synchrotron Light Technol, Ctr Struct Mol Biol, Campinas, SP, Brazil
dc.description.affiliationFFCLRP USP, Dept Chem, Ribeirao Preto, SP, Brazil
dc.description.affiliationIBILCE UNESP, Dept Phys, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespIBILCE UNESP, Dept Phys, Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 07/06755-2
dc.description.sponsorshipIdFAPESP: 07/54865
dc.description.sponsorshipIdCNPq: 307853/2006-3
dc.description.sponsorshipIdCNPq: 473997/2007-0
dc.description.sponsorshipIdCNPq: 471192/2007-4
dc.format.extent1116-1119
dc.identifierhttp://dx.doi.org/10.1107/S1744309109037567
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Malden: Wiley-blackwell Publishing, Inc, v. 65, p. 1116-1119, 2009.
dc.identifier.doi10.1107/S1744309109037567
dc.identifier.fileWOS000271421800010.pdf
dc.identifier.issn1744-3091
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
dc.identifier.urihttp://hdl.handle.net/11449/22100
dc.identifier.wosWOS:000271421800010
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleProduction, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania majoren
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
unesp.author.lattes9162508978945887[4]
unesp.author.orcid0000-0003-2460-1145[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
WOS000271421800010.pdf
Tamanho:
211.37 KB
Formato:
Adobe Portable Document Format
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: