Development of SCAR marker linked to stem canker resistance gene in soybean

dc.contributor.authorGavioli, Eduardo Antonio
dc.contributor.authorDi Mauro, Antonio Orlando [UNESP]
dc.contributor.authorCenturion, Maria Aparecida Pessoa da Cruz [UNESP]
dc.contributor.authorDi Mauro, Sônia Marli Zingaretti [UNESP]
dc.contributor.institutionInstituto Taquaritinguense de Ensino Superior (ITES)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:22:29Z
dc.date.available2014-05-27T11:22:29Z
dc.date.issued2007-06-01
dc.description.abstractStem canker caused by the fungus Diaporthe phaseolorum f. sp. meridionalis is a disease that limits soybean cultivation. Phenotypic evaluations aiming at disease resistance require labor-intensive processes, as for instance handling and transport of phytopathogens. The use of DNA markers in the selective procedures eases certain phases, besides being practical, safe and reliable. A RAPD fragment of 588pb was identified among bulks of resistant and susceptible plants in the cross BR92-15454 (R) x IAC-11 (S). Through co-segregation, the distance between the resistance locus and the fragment was estimated at 7.4 ± 2.1 cM, with a Lodmax. of 23.072 (first year) and at 6.0 ± 3.4 cM with a Lodmax. of 7.806 (second year). The fragment was converted into a SCAR marker and digested with enzyme Hinc II, which made the classification in homozygous resistant, heterozygous resistant and susceptible plants possible. This SCAR marker is suitable for use in the improvement program conducted in Jaboticabal.en
dc.description.affiliationDepartamento de Agronomia Instituto Taquaritinguense de Ensino Superior (ITES), Praça Dr. Horácio Ramalho, 159-187, 15.900-000, Taquaritinga, SP
dc.description.affiliationDepartamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP
dc.description.affiliationDepartamento de Tecnologia UNESP
dc.description.affiliationUnespDepartamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP
dc.description.affiliationUnespDepartamento de Tecnologia UNESP
dc.format.extent133-140
dc.identifierhttp://www.sbmp.org.br/cbab/siscbab/modules/news/article.php?storyid=414
dc.identifier.citationCrop Breeding and Applied Biotechnology, v. 7, n. 2, p. 133-140, 2007.
dc.identifier.file2-s2.0-41149085550.pdf
dc.identifier.issn1518-7853
dc.identifier.lattes1275652518822095
dc.identifier.scopus2-s2.0-41149085550
dc.identifier.urihttp://hdl.handle.net/11449/69709
dc.identifier.wosWOS:000254705500004
dc.language.isoeng
dc.relation.ispartofCrop Breeding and Applied Biotechnology
dc.relation.ispartofsjr0,609
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAssisted selection
dc.subjectDiaporthe phaseolorum f. sp. meridionalis
dc.subjectGlycine max
dc.subjectRAPD
dc.subjectDiaporthe phaseolorum
dc.subjectFungi
dc.titleDevelopment of SCAR marker linked to stem canker resistance gene in soybeanen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/cbab/paboutj.htm
unesp.author.lattes1275652518822095[2]
unesp.author.orcid0000-0001-6755-4153[4]
unesp.author.orcid0000-0003-1662-5745[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentProdução Vegetal - FCAVpt

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