Development of SCAR marker linked to stem canker resistance gene in soybean
dc.contributor.author | Gavioli, Eduardo Antonio | |
dc.contributor.author | Di Mauro, Antonio Orlando [UNESP] | |
dc.contributor.author | Centurion, Maria Aparecida Pessoa da Cruz [UNESP] | |
dc.contributor.author | Di Mauro, Sônia Marli Zingaretti [UNESP] | |
dc.contributor.institution | Instituto Taquaritinguense de Ensino Superior (ITES) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-27T11:22:29Z | |
dc.date.available | 2014-05-27T11:22:29Z | |
dc.date.issued | 2007-06-01 | |
dc.description.abstract | Stem canker caused by the fungus Diaporthe phaseolorum f. sp. meridionalis is a disease that limits soybean cultivation. Phenotypic evaluations aiming at disease resistance require labor-intensive processes, as for instance handling and transport of phytopathogens. The use of DNA markers in the selective procedures eases certain phases, besides being practical, safe and reliable. A RAPD fragment of 588pb was identified among bulks of resistant and susceptible plants in the cross BR92-15454 (R) x IAC-11 (S). Through co-segregation, the distance between the resistance locus and the fragment was estimated at 7.4 ± 2.1 cM, with a Lodmax. of 23.072 (first year) and at 6.0 ± 3.4 cM with a Lodmax. of 7.806 (second year). The fragment was converted into a SCAR marker and digested with enzyme Hinc II, which made the classification in homozygous resistant, heterozygous resistant and susceptible plants possible. This SCAR marker is suitable for use in the improvement program conducted in Jaboticabal. | en |
dc.description.affiliation | Departamento de Agronomia Instituto Taquaritinguense de Ensino Superior (ITES), Praça Dr. Horácio Ramalho, 159-187, 15.900-000, Taquaritinga, SP | |
dc.description.affiliation | Departamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP | |
dc.description.affiliation | Departamento de Tecnologia UNESP | |
dc.description.affiliationUnesp | Departamento de Produção Vegetal Faculdade de Ciências Agrárias e Veterinárias (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n, 14.884-900, Jaboticabal, SP | |
dc.description.affiliationUnesp | Departamento de Tecnologia UNESP | |
dc.format.extent | 133-140 | |
dc.identifier | http://www.sbmp.org.br/cbab/siscbab/modules/news/article.php?storyid=414 | |
dc.identifier.citation | Crop Breeding and Applied Biotechnology, v. 7, n. 2, p. 133-140, 2007. | |
dc.identifier.file | 2-s2.0-41149085550.pdf | |
dc.identifier.issn | 1518-7853 | |
dc.identifier.lattes | 1275652518822095 | |
dc.identifier.scopus | 2-s2.0-41149085550 | |
dc.identifier.uri | http://hdl.handle.net/11449/69709 | |
dc.identifier.wos | WOS:000254705500004 | |
dc.language.iso | eng | |
dc.relation.ispartof | Crop Breeding and Applied Biotechnology | |
dc.relation.ispartofsjr | 0,609 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Assisted selection | |
dc.subject | Diaporthe phaseolorum f. sp. meridionalis | |
dc.subject | Glycine max | |
dc.subject | RAPD | |
dc.subject | Diaporthe phaseolorum | |
dc.subject | Fungi | |
dc.title | Development of SCAR marker linked to stem canker resistance gene in soybean | en |
dc.type | Artigo | |
dcterms.license | http://www.scielo.br/revistas/cbab/paboutj.htm | |
unesp.author.lattes | 1275652518822095[2] | |
unesp.author.orcid | 0000-0001-6755-4153[4] | |
unesp.author.orcid | 0000-0003-1662-5745[2] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal | pt |
unesp.department | Produção Vegetal - FCAV | pt |
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