Improving the cytoplasmic maturation of bovine oocytes matured in vitro with intracellular and/or extracellular antioxidants is not associated with increased rates of embryo development

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dc.contributor.authorRocha-Frigoni, Nathália A.S. [UNESP]
dc.contributor.authorLeão, Beatriz C.S. [UNESP]
dc.contributor.authorDall'Acqua, Priscila Chediek [UNESP]
dc.contributor.authorMingoti, Gisele Z. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:44:01Z
dc.date.available2018-12-11T16:44:01Z
dc.date.issued2016-11-01
dc.description.abstractThe production of reactive oxygen species (ROS) is a normal process that occurs in the cellular mitochondrial respiratory chain. However, an increase in ROS levels during in vitro production of bovine embryos induces oxidative stress, leading to failed embryonic development. Therefore, we investigated whether supplementation of IVM medium with intracellular (cysteine and cysteamine; C + C) and/or extracellular (catalase; CAT) antioxidants improves the culture system, affects the mitochondrial membrane potential, affects the intracellular levels of ROS and glutathione (GSH) in the bovine oocytes at the end of maturation, and thereby affects the subsequent embryonic development. At the end of IVM, the metaphase II rates were unaffected by the treatments (76.7 ± 1.7% to 80.6 ± 5.2%; P > 0.05). The intracellular ROS levels, expressed in arbitrary fluorescence units, found in the oocytes treated with intracellular antioxidants (C + C and C + C + CAT groups; 1.06, averaged) were as low as those observed in immature oocytes (0 hour: 1.00 ± 0.12). Among mature oocytes, higher (P < 0.05) ROS levels were found in the control group (1.91 ± 0.10) when compared to the ROS levels found in oocytes treated with antioxidants. Intracellular GSH levels in all groups were lower (0.17 ± 0.09 to 0.51 ± 0.05; P < 0.05) than those in immature oocytes (1.00 ± 0.08), although GSH levels in the C + C group (0.51 ± 0.05) were greater (P < 0.05) than in the control, CAT, and C + C + CAT groups (0.23; averaged). The mitochondrial membrane potential in all groups was improved (1.6; averaged; P < 0.05) compared to the membrane potential observed in the immature oocytes (1.00 ± 0.05), with the exception of the C + C group (0.94 ± 0.03). There was no effect (P > 0.05) of antioxidant supplementation on embryonic development to the blastocyst stage (36.1%; averaged); however, there was an increased tendency (P = 0.0689) to obtain a higher blastocyst rate for the C + C + CAT group (47.5 ± 5.6%) compared to the control group (29.9 ± 4.8%). In conclusion, despite improvements in specific parameters of cytoplasmic maturation, the addition of intracellular and/or extracellular antioxidants during IVM did not affect embryo development.en
dc.description.affiliationLaboratory of Reproductive Physiology Department of Animal Health School of Veterinary Medicine UNESP–Universidade Estadual Paulista
dc.description.affiliationPost-Graduation Program in Veterinary Medicine Department of Animal Reproduction School of Agrarian and Veterinarian Sciences UNESP–Universidade Estadual Paulista
dc.description.affiliationUnespLaboratory of Reproductive Physiology Department of Animal Health School of Veterinary Medicine UNESP–Universidade Estadual Paulista
dc.description.affiliationUnespPost-Graduation Program in Veterinary Medicine Department of Animal Reproduction School of Agrarian and Veterinarian Sciences UNESP–Universidade Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #2013/07382-6
dc.description.sponsorshipIdFAPESP: (#2012/10883-8
dc.format.extent1897-1905
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2016.06.009
dc.identifier.citationTheriogenology, v. 86, n. 8, p. 1897-1905, 2016.
dc.identifier.doi10.1016/j.theriogenology.2016.06.009
dc.identifier.file2-s2.0-84991096899.pdf
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-84991096899
dc.identifier.urihttp://hdl.handle.net/11449/169019
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectExtracellular antioxidants
dc.subjectIntracellular antioxidants
dc.subjectMitochondrial potential
dc.subjectOocyte competence
dc.subjectOxidative status
dc.titleImproving the cytoplasmic maturation of bovine oocytes matured in vitro with intracellular and/or extracellular antioxidants is not associated with increased rates of embryo developmenten
dc.typeArtigo
unesp.author.orcid0000-0002-3059-4458 0000-0002-3059-4458[4]

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