High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments

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dc.contributor.authorBrun, Antonio
dc.contributor.authorPrice, Edwin R.
dc.contributor.authorGontero-Fourcade, Manuel N.
dc.contributor.authorFernandez-Marinone, Guido
dc.contributor.authorCruz-Neto, Ariovaldo P. [UNESP]
dc.contributor.authorKarasov, William H.
dc.contributor.authorCaviedes-Vidal, Enrique
dc.contributor.institutionConsejo Invest Cient & Tecn
dc.contributor.institutionUniv Wisconsin
dc.contributor.institutionUniv Nacl San Luis
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:55:54Z
dc.date.available2015-03-18T15:55:54Z
dc.date.issued2014-09-01
dc.description.abstractWater-soluble nutrients are absorbed by the small intestine via transcellular and paracellular mechanisms. Based on a few previous studies, the capacity for paracellular nutrient absorption seems greater in flying mammals than in nonflying mammals, but there has been little investigation of the mechanisms driving this difference. Therefore, we studied three species each of bats (Artibeus lituratus, Sturnira lilium and Carollia perspicillata) and nonflying mammals (Akodon montensis, Mus musculus and Rattus norvegicus). Using standard pharmacokinetic techniques in intact animals, we confirmed the greater paracellular nutrient absorption in the fliers, comparing one species in each group. Then we conducted in situ intestinal perfusions on individuals of all species. In both approaches, we measured the absorption of 3OMD-glucose, a nonmetabolizable glucose analog absorbed both paracellularly and transcellularly, as well as L-arabinose, which has no mediated transport. Fractional absorption of L-arabinose was three times higher in the bat (S. lilium: 1.2 +/- 0.24) than in the rodent (A. montensis: 0.35 +/- 0.04), whereas fractional absorption of 3OMD-glucose was complete in both species (1.46 +/- 0.4 and 0.97 +/- 0.12, respectively). In agreement, bats exhibited two to 12 times higher L-arabinose clearance per square centimeter nominal surface area than rodents in intestinal perfusions. Using L-arabinose, we estimated that the contribution of the paracellular pathway to total glucose absorption was higher in all three bats (109-137%) than in the rodents (13-39%). These findings contribute to an emerging picture that reliance on the paracellular pathway for nutrient absorption is much greater in bats relative to nonflying mammals and that this difference is driven by differences in intestinal permeability to nutrient-sized molecules.en
dc.description.affiliationConsejo Invest Cient & Tecn, Inst Multidisciplinario Invest Biol San Luis, Lab Biol Integrativa, RA-5700 San Luis, Argentina
dc.description.affiliationUniv Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA
dc.description.affiliationUniv Nacl San Luis, Dept Bioquim, RA-5700 San Luis, Argentina
dc.description.affiliationUniv Nacl San Luis, Dept Ciencias Biol, RA-5700 San Luis, Argentina
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Zool, BR-13506910 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Zool, BR-13506910 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNational Science Foundation
dc.description.sponsorshipAgencia Nacional de Promocion Cientifica y Tecnologica (FONCYT)
dc.description.sponsorshipCompany of Biologists
dc.description.sponsorshipUniversidad Nacional de San Luis
dc.description.sponsorshipDepartment of Forest and Wildlife Ecology at the University of Wisconsin-Madison
dc.description.sponsorshipIdFAPESP: 12/04610-5
dc.description.sponsorshipIdNational Science FoundationIOS-1025886
dc.description.sponsorshipIdAgencia Nacional de Promocion Cientifica y Tecnologica (FONCYT)PICT2007 01320
dc.description.sponsorshipIdUniversidad Nacional de San LuisCyT 9502
dc.format.extent3311-3317
dc.identifierhttp://dx.doi.org/10.1242/jeb.104927
dc.identifier.citationJournal Of Experimental Biology. Cambridge: Company Of Biologists Ltd, v. 217, n. 18, p. 3311-3317, 2014.
dc.identifier.doi10.1242/jeb.104927
dc.identifier.issn0022-0949
dc.identifier.urihttp://hdl.handle.net/11449/117348
dc.identifier.wosWOS:000342506100022
dc.language.isoeng
dc.publisherCompany Of Biologists Ltd
dc.relation.ispartofJournal Of Experimental Biology
dc.relation.ispartofjcr3.179
dc.relation.ispartofsjr1,611
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectIntestineen
dc.subjectMediated absorptionen
dc.subjectParacellular absorptionen
dc.subjectArabinoseen
dc.subjectFlighten
dc.subjectBatsen
dc.subjectIntestinal perfusionen
dc.subjectAkodon montensisen
dc.subjectSturnira liliumen
dc.subjectArtibeus lituratusen
dc.subjectCarollia perspicillataen
dc.subjectMus musculusen
dc.subjectRattus norvegicusen
dc.titleHigh paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segmentsen
dc.typeArtigo
dcterms.rightsHolderCompany Of Biologists Ltd
unesp.author.lattes5758081094133626[5]
unesp.author.orcid0000-0003-4526-4969[7]
unesp.author.orcid0000-0001-6042-7020[2]
unesp.author.orcid0000-0001-5270-7276[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Artigos - Zoologia - IBRC