The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts

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dc.contributor.authorOlímpio, Regiane Marques Castro [UNESP]
dc.contributor.authorMoretto, Fernanda Cristina Fontes [UNESP]
dc.contributor.authorDe Sibio, Maria Teresa [UNESP]
dc.contributor.authorde Oliveira, Miriane [UNESP]
dc.contributor.authorMathias, Lucas Solla [UNESP]
dc.contributor.authorGonçalves, Bianca Mariani [UNESP]
dc.contributor.authorDeprá, Igor Carvalho [UNESP]
dc.contributor.authorTilli, Helena Paim. [UNESP]
dc.contributor.authorRodrigues, Bruna Moretto [UNESP]
dc.contributor.authorSaraiva, Patrícia Pinto [UNESP]
dc.contributor.authorMaria, Durvanei Augusto
dc.contributor.authorNogueira, Célia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionButantan Institute
dc.date.accessioned2019-10-06T16:34:57Z
dc.date.available2019-10-06T16:34:57Z
dc.date.issued2019-08-15
dc.description.abstractTriiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism and associated E2 in human cells. AIMS: This study evaluated the effects of the physiological concentration of E2 (10 nM), alone or in association with physiological (1 nM) and supraphysiological (10 nM) concentrations of T3, on RANKL and OPG gene expression in human osteoblasts. MAIN METHODS: Alkaline phosphatase and osteocalcin assays were performed to verify the presence of mature osteoblasts. After mimicking the experimental hyperthyroidism in osteoblasts untreated or treated with E2, RANKL and OPG gene expression was analyzed by real-time PCR and protein expression by western Blot and ELISA. Alizarin Red staining analyzed the amount of bone matrix after hormonal treatments. KEY FINDINGS: E2 enhanced the gene expression of OPG when associated with 1 nM and 10 nM T3. E2 was able to restore the bone matrix after an initial decrease using 1 nM and 10 nM T3. The protective effect of E2 on the RANKL and OPG signaling pathway was demonstrated. E2 restored the bone matrix induced by experimental hyperthyroidism. SIGNIFICANCE: The data highlight the importance of E2 to maintain OPG expression and osteoblast activity against possible loss of bone mass, especially in conditions where T3 is in excess.en
dc.description.affiliationDepartment of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESP
dc.description.affiliationLaboratory Biochemistry and Biophysics Butantan Institute, 1500, Avenue Vital Brazil
dc.description.affiliationUnespDepartment of Internal Medicine Botucatu Medical School Univ Estadual Paulista - UNESP
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2014/16406-9
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2019.116556
dc.identifier.citationLife Sciences, v. 231.
dc.identifier.doi10.1016/j.lfs.2019.116556
dc.identifier.issn1879-0631
dc.identifier.issn0024-3205
dc.identifier.scopus2-s2.0-85067341769
dc.identifier.urihttp://hdl.handle.net/11449/189257
dc.language.isoeng
dc.relation.ispartofLife Sciences
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectEstrogen
dc.subjectOsteoblast
dc.subjectRANKL and OPG
dc.subjectStem cells
dc.subjectTriiodothyronine
dc.titleThe importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblastsen
dc.typeArtigo
unesp.author.lattes7607038776901890[12]
unesp.author.orcid0000-0002-4014-0660[12]

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