Evaluation of serological and molecular tests used to identify Toxoplasma gondii infection in pregnant women attended in a public health service in São Paulo state, Brazil
Murata, Fernando Henrique Antunes
Ferreira, Marina Neves [UNESP]
Pereira-Chioccola, Vera Lucia
Spegiorin, Lígia Cosentino Junqueira Franco
Meira-Strejevitch, Cristina da Silva
Silveira‐Carvalho, Aparecida Perpétuo
de Mattos, Luiz Carlos
Brandão de Mattos, Cinara Cássia
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Toxoplasmosis during pregnancy can have severe consequences. The use of sensitive and specific serological and molecular methods is extremely important for the correct diagnosis of the disease. We compared the ELISA and ELFA serological methods, conventional PCR (cPCR), Nested PCR and quantitative PCR (qPCR) in the diagnosis of Toxoplasma gondii infection in pregnant women without clinical suspicion of toxoplasmosis (G1 = 94) and with clinical suspicion of toxoplasmosis (G2 = 53). The results were compared using the Kappa index, and the sensitivity, specificity, positive predictive value and negative predictive value were calculated. The results of the serological methods showed concordance between the ELISA and ELFA methods even though ELFA identified more positive cases than ELISA. Molecular methods were discrepant with cPCR using B22/23 primers having greater sensitivity and lower specificity compared to the other molecular methods.
Gestational toxoplasmosis, Molecular diagnosis, Polymerase chain reaction, Serology, Toxoplasma gondii, Toxoplasmosis
Diagnostic Microbiology and Infectious Disease, v. 89, n. 1, p. 13-19, 2017.