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Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)

dc.contributor.authorFanali, Lara Zácari [UNESP]
dc.contributor.authorDe Oliveira, Classius [UNESP]
dc.contributor.authorSturve, Joachim
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Gothenburg
dc.date.accessioned2021-06-25T11:00:45Z
dc.date.available2021-06-25T11:00:45Z
dc.date.issued2021-01-01
dc.description.abstractFish have defense systems that are capable of repairing damages caused by xenobiotics like benzo[a]pyrene (BaP), so the aims of this study were to identify BaP toxicity in melanomacrophages (MMs) cytoskeleton, evaluate the melanin area in MMs, and analyze genotoxicity. Rainbow trout juveniles (n = 24) were split in 48h and 7d treatments that received 2 mg/kg of BaP. After the experiment, blood samples were collected and liver was removed, to proceed with the analysis: EROD activity, MMs melanin area quantification, melanosomes movements, and a genotoxicity test. The results revealed increased in EROD activity after 48-h and 7-day BaP exposure. The group 7d displayed a reduction in MMs pigmented area, melanosomes aggregation, in addition to an increased frequency of micronucleus. By means of the EROD assay, it was possible to confirm the activation of BaP biotransformation system. The impairment of the melanosomes’ movements possibly by an inactivation of the protein responsible for the pigment dispersion consequently affects the melanin area and thus might negatively impact the MMs detoxification capacity. In addition to this cytotoxicity, the increased frequency of micronucleus might also indicate the genotoxicity of BaP in this important fish species.en
dc.description.affiliationGraduate Program in Animal Biology Universidade Estadual Paulista (UNESP), São José do Rio Preto
dc.description.affiliationDepartment of Biology Universidade Estadual Paulista (UNESP), São José do Rio Preto
dc.description.affiliationDepartment of Biological and Environmental Sciences University of Gothenburg, Box 463
dc.description.affiliationUnespGraduate Program in Animal Biology Universidade Estadual Paulista (UNESP), São José do Rio Preto
dc.description.affiliationUnespDepartment of Biology Universidade Estadual Paulista (UNESP), São José do Rio Preto
dc.identifierhttp://dx.doi.org/10.1007/s11356-021-14583-1
dc.identifier.citationEnvironmental Science and Pollution Research.
dc.identifier.doi10.1007/s11356-021-14583-1
dc.identifier.issn1614-7499
dc.identifier.issn0944-1344
dc.identifier.lattes5731856650217859
dc.identifier.orcid0000-0002-2029-7900
dc.identifier.scopus2-s2.0-85106454894
dc.identifier.urihttp://hdl.handle.net/11449/207771
dc.language.isoeng
dc.relation.ispartofEnvironmental Science and Pollution Research
dc.sourceScopus
dc.subjectCytoskeleton
dc.subjectEROD
dc.subjectErythrocytes abnormalities
dc.subjectLiver
dc.subjectMelanomacrophage
dc.subjectMorphological alteration
dc.titleEnzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)en
dc.typeArtigo
unesp.author.lattes5731856650217859[2]
unesp.author.orcid0000-0001-8678-6419[1]
unesp.author.orcid0000-0002-2029-7900[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBiologia - IBILCEpt

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