Functional and Morphological Reproductive Aspects in Male Rats Exposed to Di-n-Butyl Phthalate (DBP) in Utero and During Lactation

dc.contributor.authorScarano, Wellerson R. [UNESP]
dc.contributor.authorToledo, Fabiola C.
dc.contributor.authorGuerra, Marina T.
dc.contributor.authorPinheiro, Patricia F. F.
dc.contributor.authorDomeniconi, Raquel F.
dc.contributor.authorFelisbino, Sergio L.
dc.contributor.authorCampos, Silvana G. P. [UNESP]
dc.contributor.authorTaboga, Sebastiao R. [UNESP]
dc.contributor.authorKempinas, Wilma G.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionFaculdade de Medicina de Marília (FAMEMA)
dc.date.accessioned2014-05-20T13:52:09Z
dc.date.available2014-05-20T13:52:09Z
dc.date.issued2010-01-01
dc.description.abstractThe potential adverse reproductive effects, with emphasis on the epididymis, of in utero and lactational exposure to 100 mg/kg/d di-n-butyl phthalate (DBP) in adult male rat offspring were investigated. The fetal testis histopathology was also determined. The selected endpoints included reproductive organ weights, sperm motility and morphology, sperm epididymal transit time, sperm quantity in the testis and epididymis, hormonal status, fetal testis and epididymal histopathology and stereology, and androgen receptor (AR), aquaporin 9 (AQP9), and Ki-67 immunoreactivities. Pregnant females were divided into two groups: control (C) and treated (T). The treated females received DBP (100 mg/kg/d, by gavage) from gestation day (GD) 12 to postnatal day (PND) 21, while control dams received the vehicle. Some pregnant dams were killed by decapitation on GD20, and testes from male fetuses were collected for histopathogy. Male rats from other dams were killed at PND 90. Fetal testes from treated group showed Leydig-cell clusters, presence of multinucleated germinative cells, and increase of the interstitial component. Testosterone levels and reproductive organ weights were similar between the treated and control adult groups. DBP treatment did not markedly affect relative proportions of epithelial, stromal, or luminal compartments in the epididymis; sperm counts in the testis and epididymis; sperm transit time; or sperm morphology and motility in adult rats. The AR and AQP9 immunoreactivities and proliferation index were similar for the two groups. These results showed that fetal testes were affected by DBP as evidenced by testicular histopathologic alterations, but reproductive parameters and epididymal structure/function were not significantly altered in the adult animals exposed to 100 mg/kg DBP in utero and during lactation.en
dc.description.affiliationUniv Estadual Paulista, Inst Biosci, Dept Morphol, UNESP, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Grad Program Struct & Cell Biol, São Paulo, Brazil
dc.description.affiliationFAMEMA, Fac Med Marilia, Marilia, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biosci, Dept Morphol, UNESP, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, IBILCE, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.format.extent972-984
dc.identifierhttp://dx.doi.org/10.1080/15287391003751760
dc.identifier.citationJournal of Toxicology and Environmental Health-part A-current Issues. Philadelphia: Taylor & Francis Inc, v. 73, n. 13-14, p. 972-984, 2010.
dc.identifier.doi10.1080/15287391003751760
dc.identifier.issn1528-7394
dc.identifier.lattes5760560970751598
dc.identifier.lattes5481756528299469
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.orcid0000-0003-1452-5708
dc.identifier.orcid0000-0003-2938-010X
dc.identifier.urihttp://hdl.handle.net/11449/18628
dc.identifier.wosWOS:000278924900015
dc.language.isoeng
dc.publisherTaylor & Francis Inc
dc.relation.ispartofJournal of Toxicology and Environmental Health: Part A Current Issues
dc.relation.ispartofjcr2.706
dc.relation.ispartofsjr0,888
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleFunctional and Morphological Reproductive Aspects in Male Rats Exposed to Di-n-Butyl Phthalate (DBP) in Utero and During Lactationen
dc.typeArtigo
dcterms.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dcterms.rightsHolderTaylor & Francis Inc
unesp.author.lattes5760560970751598[4]
unesp.author.lattes5481756528299469[5]
unesp.author.orcid0000-0002-6682-2934[1]
unesp.author.orcid0000-0002-0970-4288[8]
unesp.author.orcid0000-0003-2938-010X[5]
unesp.author.orcid0000-0002-2112-5123[9]
unesp.author.orcid0000-0003-1452-5708[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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