VIABILITY of SPORULATED OOCYSTS of NEOSPORA CANINUM AFTER EXPOSURE TO DIFFERENT PHYSICAL and CHEMICAL TREATMENTS

dc.contributor.authorAlves Neto, Aldo F. [UNESP]
dc.contributor.authorBandini, Luciana A.
dc.contributor.authorNishi, Sandra M.
dc.contributor.authorSoares, Rodrigo M.
dc.contributor.authorDriemeier, David
dc.contributor.authorAntoniassi, Nadia A. B.
dc.contributor.authorSchares, Gereon
dc.contributor.authorGennari, Solange M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:27Z
dc.date.available2014-05-20T15:30:27Z
dc.date.issued2011-02-01
dc.description.abstractThe aim of the present study was to evaluate the viability of Neospora caninum sporulated oocysts after various chemical and physical treatments. Bioassays in gerbils and molecular techniques (PCR-RFLP) were used for identification of the oocysts shed by experimentally infected dogs. Sporulated oocysts were purified and divided into 11 treatment groups as follows: absolute ethanol for 1 hr; 20 C for 6 hr; 4 C for 6 hr; 60 C for 1 min; 100 C for 1 min; 10% formaldehyde for 1 hr; 10% ammonia for 1 hr; 2% iodine for 1 hr; 10% sodium hypochlorite for I hr; 70% ethanol for I hr; and one group was left untreated and kept as a positive control. All chemical treatments were performed at room temperature (37 C). A total of 33 gerbils, or 3 gerbils per treatment, were used for bioassays. After treatment, the oocysts were divided into aliquots of 1,000 oocysts and orally administered to gerbils. After 63 days, the gerbils were anesthetized and killed with 0.2 ml of T61; blood and tissue samples were collected for serological (IFAT and western blotting), molecular (real-time PCR), histopathology, and immunohistochemical tests. Treatments were considered effective only if all 5 detection techniques tested negative. High temperatures at 100 C for 1 min and 10% sodium hypochlorite for 1 hr were the only treatments that met this condition, effectively inactivating all oocysts.en
dc.description.affiliationUniv São Paulo, Fac Med Vet & Zootecnia, BR-05508270 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent135-139
dc.identifierhttp://dx.doi.org/10.1645/GE-2571.1
dc.identifier.citationJournal of Parasitology. Lawrence: Amer Soc Parasitologists, v. 97, n. 1, p. 135-139, 2011.
dc.identifier.doi10.1645/GE-2571.1
dc.identifier.issn0022-3395
dc.identifier.urihttp://hdl.handle.net/11449/39820
dc.identifier.wosWOS:000288056500022
dc.language.isoeng
dc.publisherAmer Soc Parasitologists
dc.relation.ispartofJournal of Parasitology
dc.relation.ispartofjcr1.395
dc.relation.ispartofsjr0,662
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleVIABILITY of SPORULATED OOCYSTS of NEOSPORA CANINUM AFTER EXPOSURE TO DIFFERENT PHYSICAL and CHEMICAL TREATMENTSen
dc.typeArtigo
dcterms.licensehttp://www.journalofparasitology.org/page/permissions
dcterms.rightsHolderAmer Soc Parasitologists

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