Brain morphophysiology of Africanized bee Apis mellifera exposed to sublethal doses of imidacloprid

Página do item simplificado
dc.contributor.authorDe Almeida Rossi, Caroline [UNESP]
dc.contributor.authorRoat, Thaisa Cristina [UNESP]
dc.contributor.authorTavares, Daiana Antonia [UNESP]
dc.contributor.authorCintra-Socolowski, Priscila [UNESP]
dc.contributor.authorMalaspina, Osmar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:04Z
dc.date.available2014-05-27T11:30:04Z
dc.date.issued2013-08-01
dc.description.abstractSeveral synthetic substances are used in agricultural areas to combat insect pests; however, the indiscriminate use of these products may affect nontarget insects, such as bees. In Brazil, one of the most widely used insecticides is imidacloprid, which targets the nervous system of insects. Therefore, the aim of this study was to evaluate the effects of chronic exposure to sublethal doses of imidacloprid on the brain of the Africanized Apis mellifera. The organs of both control bees and bees exposed to insecticide were subjected to morphological, histochemical and immunocytochemical analysis after exposure to imidacloprid, respectively, for 1, 3, 5, 7, and 10 days. In mushroom bodies of bees exposed to imidacloprid concentrations of LD50/10 and in optic lobes of bees exposed to imidacloprid concentrations of LD 50/10, LD50/100, and LD50/50, we observed the presence of condensed cells. The Feulgen reaction revealed the presence of some cells with pyknotic nuclei, whereas Xylidine Ponceau stain revealed strongly stained cells. These characteristics can indicate the occurrence of cell death. Furthermore, cells in mushroom bodies of bees exposed to imidacloprid concentrations of LD50/10 appeared to be swollen. Cell death was confirmed by immunocytochemical technique. Therefore, it was concluded that sublethal doses of imidacloprid have cytotoxic effects on exposed bee brains and that optic lobes are more sensitive to the insecticide than other regions of the brain. © 2013 Springer Science+Business Media New York.en
dc.description.affiliationDepartamento de Biologia Centro de Estudos de Insetos Sociais UNESP-Universidade Estadual Paulista, Av. 24A, 1515, Bela Vista, Rio Claro, SP 13500-900
dc.description.affiliationUnespDepartamento de Biologia Centro de Estudos de Insetos Sociais UNESP-Universidade Estadual Paulista, Av. 24A, 1515, Bela Vista, Rio Claro, SP 13500-900
dc.format.extent234-243
dc.identifierhttp://dx.doi.org/10.1007/s00244-013-9897-1
dc.identifier.citationArchives of Environmental Contamination and Toxicology, v. 65, n. 2, p. 234-243, 2013.
dc.identifier.doi10.1007/s00244-013-9897-1
dc.identifier.issn0090-4341
dc.identifier.issn1432-0703
dc.identifier.lattes7538556085505819
dc.identifier.orcid0000-0002-1650-257X
dc.identifier.scopus2-s2.0-84880324906
dc.identifier.scopus2-s2.0-84875689588
dc.identifier.urihttp://hdl.handle.net/11449/76101
dc.identifier.wosWOS:000321785600007
dc.language.isoeng
dc.relation.ispartofArchives of Environmental Contamination and Toxicology
dc.relation.ispartofjcr2.421
dc.relation.ispartofsjr0,773
dc.relation.ispartofsjr0,773
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectimidacloprid
dc.subjectbee
dc.subjectbrain
dc.subjectdose-response relationship
dc.subjectentomology
dc.subjectmorphology
dc.subjectnontarget organism
dc.subjectsublethal effect
dc.subjectanimal experiment
dc.subjectApis mellifera
dc.subjectcontrolled study
dc.subjectdrug cytotoxicity
dc.subjectFeulgen staining
dc.subjecthistochemistry
dc.subjectimmunocytochemistry
dc.subjectLD 50
dc.subjectlong term exposure
dc.subjectmushroom body
dc.subjectnonhuman
dc.subjectoptic lobe
dc.subjectpriority journal
dc.subjectBrazil
dc.subjectApoidea
dc.subjectBasidiomycota
dc.subjectHexapoda
dc.titleBrain morphophysiology of Africanized bee Apis mellifera exposed to sublethal doses of imidaclopriden
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes7538556085505819
unesp.author.orcid0000-0002-1650-257X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

Arquivos

Coleções

Artigos - Biologia - IBRC