Determinação de carbaril utilizando testes ELISA (Enzyme-linked immunosorbent assay) e CLAE com detecção por arranjo de diodos

Toscano, Ilda A. S. [UNESP]; Ribeiro, M. Lúcia [UNESP]; Rocha, Julio C. [UNESP]; Nunes, Gilvanda S.; Barceló, Damiá

Determinação de carbaril utilizando testes ELISA (Enzyme-linked immunosorbent assay) e CLAE com detecção por arranjo de diodos

Arquivos

S0100-40422000000400007.pdf (58.92 KB)

Data

2000-08-01

Autores

Editor

Sociedade Brasileira de Química

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

ELISAs have been applied to pesticide residue analysis due to their high sensitivity and selectivity. However, some ELISAs performance may be affected by matrix components. In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids. The results suggested that the high fulvic acid concentration ( ³30 mg L-1) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity. A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method. Good correlation between ELISA and HPLC-DAD results was observed.