Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle

Silva, Marcelo Santos da [UNESP]; Monteiro, Jomar Patricio; Nunes, Vinicius Santana [UNESP]; Vasconcelos, Elton Jose; Perez, Arina Marina [UNESP]; Freitas-Junior, Lucio de Holanda; Elias, Maria Carolina; Nogueira Cano, Maria Isabel [UNESP]

Leishmania amazonensis Promastigotes Present Two Distinct Modes of Nucleus and Kinetoplast Segregation during Cell Cycle

Arquivos

WOS000327539800128.pdf (1.77 MB)

Data

2013-11-21

Autores

Silva, Marcelo Santos da

Monteiro, Jomar Patricio

Nunes, Vinicius Santana

Vasconcelos, Elton Jose

Perez, Arina Marina

Freitas-Junior, Lucio de Holanda

Elias, Maria Carolina

Nogueira Cano, Maria Isabel

Editor

Public Library Science

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Here, we show the morphological events associated with organelle segregation and their timing in the cell cycle of a reference strain of Leishmania (L.) amazonensis promastigotes, the main causative agent of Tegumentary leishmaniasis in the Americas. We show evidences that during the cell cycle, L. amazonensis promastigotes present two distinct modes of nucleus and kinetoplast segregation, which occur in different temporal order in different proportions of cells. We used DAPI-staining and EdU-labeling to monitor the segregation of DNA-containing organelles and DNA replication in wild-type parasites. The emergence of a new flagellum was observed using a specific monoclonal antibody. The results show that L. amazonensis cell cycle division is peculiar, with 65% of the dividing cells duplicating the kinetoplast before the nucleus, and the remaining 35% doing the opposite or duplicating both organelles concomitantly. In both cases, the new flagellum appeared during S to G2 phase in 1N1K cells and thus before the segregation of both DNA-containing organelles; however, we could not determine the exact timing of flagellar synthesis. Most of these results were confirmed by the synchronization of parasites using hydroxyurea. Altogether, our data show that during the cell cycle of L. amazonensis promastigotes, similarly to L. donovani, the segregation of nucleus and kinetoplast do not follow a specific order, especially when compared to other trypanosomatids, reinforcing the idea that this characteristic seems to be species-specific and may represent differences in cellular biology among members of the Leishmania genus.