Cattle-Derived Microsatellite Markers to Study the Water Buffalo Genome
Nenhuma Miniatura disponível
Data
2013-01-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Int Buffalo Information Ctr
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
Microsatellites are well-known DNA markers used in a variety of studies such as genome mapping, genetic diversity analysis, genetic conservation and phylogenetic studies. Although microsatellites are important markers, their development and characterization demands extensive time and high cost. Thus, before new markers are developed for a particular species, it is worthwhile to test the available markers from related species. In the present study, we evaluate cattle-derived microsatellite markers for genetic studies of water buffalo. Eighty-five percents of a total of 120 microsatellite markers were optimized using buffalo DNA (Bubalus bubalis). The results showed in this paper were also deposited in the National Center for Biological Information database (NCBI) (ProbeDB and UniSTS) for use in population genetic studies of buffalo by the scientific community. The use of heterologous primers significantly reduces the cost of developing specific markers for buffalo, providing a useful short cut for the genetic population analysis and gene mapping studies.
Descrição
Palavras-chave
Idioma
Inglês
Como citar
Buffalo Bulletin. Bangkok: Int Buffalo Information Ctr, v. 32, p. 681-685, 2013.