Scanning Electron Microscopy of Male Terminalia and Its Application to Species Recognition and Phylogenetic Reconstruction in the Drosophila saltans Group

dc.contributor.authorJorge Souza, Tiago Alves
dc.contributor.authorNoll, Fernando Barbosa [UNESP]
dc.contributor.authorMelara de Campos Bicudo, Hermione Elly [UNESP]
dc.contributor.authorMadi-Ravazzi, Lilian [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:56:01Z
dc.date.available2015-03-18T15:56:01Z
dc.date.issued2014-06-10
dc.description.abstractThe Drosophila saltans group consists of five subgroups and 21 species, most of which have been identified only by morphological aspects of the male terminalia revealed by drawings using a camera lucida and a bright-field microscope. However, several species in the group, mainly those included in the saltans subgroup, are difficult to differentiate using only these characteristics. In this study, we used scanning electron microscopy (SEM) to analyze 19 structures of the male terminalia in 10 species from the five saltans subgroups. Among these structures, nine could be identified only through SEM analysis. We aimed to find other characteristics useful for morphological recognition of these species and to use these characteristics for phylogenetic reconstruction. These morphological differences enabled us to effectively distinguish among sibling species. These findings confirmed the monophyly of this group as previously determined in evolutionary studies based on other markers. The single most parsimonious tree (CI = 87 and RI = 90) indicated that the cordata subgroup is the most basal lineage and the saltans subgroup is the most apical lineage, as shown in earlier studies based on morphological data. However, our findings differed somewhat from these studies with respect to the phylogenetic relationships of species in the saltans group indicating that this group is still a puzzle that remains to be deciphered.en
dc.description.affiliationUniv Sao Paulo, Dept Genet, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationSao Paulo State Univ, UNESP IBILCE, Dept Bot & Zool, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationSao Paulo State Univ, UNESP IBILCE, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, UNESP IBILCE, Dept Bot & Zool, Sao Jose Do Rio Preto, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, UNESP IBILCE, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 07/07180-3
dc.format.extent12
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0097156
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 6, 12 p., 2014.
dc.identifier.doi10.1371/journal.pone.0097156
dc.identifier.fileWOS000340947700009.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes8347131704153687
dc.identifier.orcid0000-0003-0207-1067
dc.identifier.urihttp://hdl.handle.net/11449/117388
dc.identifier.wosWOS:000340947700009
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleScanning Electron Microscopy of Male Terminalia and Its Application to Species Recognition and Phylogenetic Reconstruction in the Drosophila saltans Groupen
dc.typeArtigo
dcterms.rightsHolderPublic Library Science
unesp.author.lattes8347131704153687[2]
unesp.author.orcid0000-0003-0207-1067[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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