Systemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritis

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dc.contributor.authorIshikawa, Larissa Lumi Watanabe [UNESP]
dc.contributor.authorColavite, Priscila Maria [UNESP]
dc.contributor.authorFraga-Silva, Thais Fernanda De Campos [UNESP]
dc.contributor.authorMimura, Luiza Ayumi Nishiyama [UNESP]
dc.contributor.authorFran�a, Thais Graziela Doneg� [UNESP]
dc.contributor.authorZorzella-Pezavento, Sofia Fernanda Gon�alves [UNESP]
dc.contributor.authorChiuso-Minicucci, Fernanda [UNESP]
dc.contributor.authorMarcolino, Larissa Doddi [UNESP]
dc.contributor.authorMarques, Camila
dc.contributor.authorIkoma, Maura Rosane Valerio
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAmaral Carvalho Foundation
dc.date.accessioned2018-12-11T17:03:13Z
dc.date.available2018-12-11T17:03:13Z
dc.date.issued2016-01-01
dc.description.abstractThis study was undertaken to evaluate the prophylactic potential of proteoglycan (PG) administration in experimental arthritis. Female BALB/c retired breeder mice received two (2xPG50 and 2xPG100 groups) or three (3xPG50 group) intraperitoneal doses of bovine PG (50 μg or 100 μg) every three days. A week later the animals were submitted to arthritis induction by immunization with three i.p. doses of bovine PG associated with dimethyldioctadecylammonium bromide adjuvant at intervals of 21 days. Disease severity was daily assessed after the third dose by score evaluation. The 3xPG50 group showed significant reduction in prevalence and clinical scores. This protective effect was associated with lower production of IFN-γ and IL-17 and increased production of IL-5 and IL-10 by spleen cells restimulated in vitro with PG. Even though previous PG administration restrained dendritic cells maturation this procedure did not alter the frequency of regulatory Foxp3+ T cells. Lower TNF-α and IL-6 levels and higher expression of ROR-γ and GATA-3 were detected in the paws of protected animals. A delayed-type hypersensitivity reaction confirmed specific tolerance induction. Taken together, these results indicate that previous PG inoculation determines a specific tolerogenic effect that is able to decrease severity of subsequently induced arthritis.en
dc.description.affiliationDepartment of Microbiology and Immunology Institute of Biosciences of Botucatu S�o Paulo State University (UNESP)
dc.description.affiliationDepartment of Pathology Botucatu Medical School
dc.description.affiliationLaboratory of Flow Cytometry Amaral Carvalho Foundation
dc.description.affiliationUnespDepartment of Microbiology and Immunology Institute of Biosciences of Botucatu S�o Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Pathology Botucatu Medical School
dc.identifierhttp://dx.doi.org/10.1155/2016/6765134
dc.identifier.citationJournal of Immunology Research, v. 2016.
dc.identifier.doi10.1155/2016/6765134
dc.identifier.file2-s2.0-84973101029.pdf
dc.identifier.issn2314-7156
dc.identifier.issn2314-8861
dc.identifier.scopus2-s2.0-84973101029
dc.identifier.urihttp://hdl.handle.net/11449/173034
dc.language.isoeng
dc.relation.ispartofJournal of Immunology Research
dc.relation.ispartofsjr1,352
dc.relation.ispartofsjr1,352
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleSystemic Administration of Proteoglycan Protects BALB/c Retired Breeder Mice from Experimental Arthritisen
dc.typeArtigo
unesp.author.lattes4977572416129527[11]
unesp.author.orcid0000-0003-4557-3331[11]

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