Solubilization of membrane-bound matrix-induced alkaline phosphatase with polyoxyethylene 9-lauryl ether (polidocanol): Purification and metalloenzyme properties

dc.contributor.authorCiancaglini, P.
dc.contributor.authorPizauro, J. M. [UNESP]
dc.contributor.authorRezende, A. A.
dc.contributor.authorRezende, L. A.
dc.contributor.authorLeone, F. A.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:53:48Z
dc.date.available2022-04-28T19:53:48Z
dc.date.issued1990-01-01
dc.description.abstract1. 1. Matrix-induced alkaline phosphatase prepared from rat osseous plate was solubilized with polidocanol and purified on a Sephacryl S-300 column. 2. 2. Purified solubilized alkaline phosphatase has a molecular weight of ca 115,000 and bind one magnesium and two zinc ions. At least 110 detergent molecules are bound to each enzyme molecule. 3. 3. Solubilization and purification procedures did not destroy the ability of the enzyme to hydrolyze adenosine-5'-triphosphate, p-nitrophenylphosphate, pyrophosphate and bis p-nitrophenylphosphate. 4. 4. Magnesium, manganese and cobalt ions are stimulators of PNPPase activity of solubilized enzyme whereas calcium and zinc ions are inhibitors. © 1990.en
dc.description.affiliationDepartamento de Química, Faculdade de Filosofia, Ciências e Letras-USP, 14049 Ribeirão Preto, SP
dc.description.affiliationDepartamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias-UNESP., 14870 Jaboticabal, SP
dc.description.affiliationUnespDepartamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias-UNESP., 14870 Jaboticabal, SP
dc.format.extent385-392
dc.identifierhttp://dx.doi.org/10.1016/0020-711X(90)90141-O
dc.identifier.citationInternational Journal of Biochemistry, v. 22, n. 4, p. 385-392, 1990.
dc.identifier.doi10.1016/0020-711X(90)90141-O
dc.identifier.issn0020-711X
dc.identifier.scopus2-s2.0-0025248892
dc.identifier.urihttp://hdl.handle.net/11449/223916
dc.language.isoeng
dc.relation.ispartofInternational Journal of Biochemistry
dc.sourceScopus
dc.titleSolubilization of membrane-bound matrix-induced alkaline phosphatase with polyoxyethylene 9-lauryl ether (polidocanol): Purification and metalloenzyme propertiesen
dc.typeArtigo
Arquivos