Phenotypic markers of oral keratinocytes seeded on two distinct 3D oral mucosa models

dc.contributor.authorBasso, Fernanda G. [UNESP]
dc.contributor.authorPansani, Taisa N. [UNESP]
dc.contributor.authorMarcelo, Cyntia L.
dc.contributor.authorde Souza Costa, Carlos Alberto [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorFeinberg, Stephen E.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Michighan
dc.date.accessioned2018-12-11T17:36:59Z
dc.date.available2018-12-11T17:36:59Z
dc.date.issued2018-09-01
dc.description.abstractThis study validates the use of a full-thickness oral mucosa model for in vitro studies with a collagen type I matrix, by comparison of this model with two other 3D oral mucosa models: human-sourced and porcine acellular dermal matrices (AlloDerm®/Strattice®, respectively). For the collagen matrix model, gingival fibroblasts were seeded either onto the dermal side of the AlloDerm® and Strattice® matrices or within the collagen matrices in complete culture medium (DMEM). For all scaffolds, DMEM was replaced every 24 h up to 72 h. For the full-thickness oral mucosa models, 72 h after fibroblast seeding, oral keratinocytes were seeded on the epidermal sides of AlloDerm® and Strattice® matrices or collagen matrices. All matrices and models were subjected to histological analysis, complementing phenotypic characterization by evaluation of glucose consumption, cell proliferation, gene expression and synthesis of growth factors. A higher fibroblast ratio was observed for the collagen matrix, in which the distribution of gingival fibroblasts was also more homogeneous. Metabolism, proliferation, and gene expression and synthesis of VEGF of these cells were also increased for the collagen matrix. All matrices provided a suitable substrate for oral keratinocytes adhesion, proliferation, and phenotypic expression; however, higher proliferation, stratification, and differentiation were noted when oral keratinocytes were seeded on the dermal matrices.en
dc.description.affiliationUniversidade Estadual Paulista (Unesp) Araraquara Dental School
dc.description.affiliationUniversity of Michighan
dc.description.affiliationUnespUniversidade Estadual Paulista (Unesp) Araraquara Dental School
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/17947-8
dc.description.sponsorshipIdFAPESP: 2013/05879-0
dc.description.sponsorshipIdFAPESP: 2014/06057-7
dc.format.extent34-39
dc.identifierhttp://dx.doi.org/10.1016/j.tiv.2018.04.015
dc.identifier.citationToxicology in Vitro, v. 51, p. 34-39.
dc.identifier.doi10.1016/j.tiv.2018.04.015
dc.identifier.file2-s2.0-85046654521.pdf
dc.identifier.issn1879-3177
dc.identifier.issn0887-2333
dc.identifier.scopus2-s2.0-85046654521
dc.identifier.urihttp://hdl.handle.net/11449/179844
dc.language.isoeng
dc.relation.ispartofToxicology in Vitro
dc.relation.ispartofsjr0,931
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCell culture techniques
dc.subjectGingival fibroblasts
dc.subjectOrganotypic cell culture
dc.titlePhenotypic markers of oral keratinocytes seeded on two distinct 3D oral mucosa modelsen
dc.typeArtigo
unesp.author.lattes4517484241515548[4]
unesp.author.orcid0000-0002-7170-2371[1]
unesp.author.orcid0000-0002-7455-6867[4]

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