Cyclodextrin production by bacillus lehensis isolated from cassava starch: Characterisation of a novel enzyme

dc.contributor.authorBlanco, Kate Cristina [UNESP]
dc.contributor.authorDe Moraes, Flávio Faria
dc.contributor.authorBernardi, Natalia Sozza [UNESP]
dc.contributor.authorVettori, Mary Helen Palmuti Braga [UNESP]
dc.contributor.authorMonti, Rubens [UNESP]
dc.contributor.authorContiero, Jonas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.date.accessioned2018-12-11T16:55:35Z
dc.date.available2018-12-11T16:55:35Z
dc.date.issued2014-03-04
dc.description.abstractThe properties of a previously unknown enzyme, denominated cyclodextrin glycosyltransferase, produced from Bacillus lehensis, were evaluated using affinity chromatography for protein purification. Enzyme characteristics (optimum pH and temperature; pH and temperature stability), the influence of substances on the enzyme activity, enzyme kinetics, and cyclodextrin production were analysed. Cyclodextrin glycosyltransferase was purified up to 320.74-fold by affinity chromatography using β-cyclodextrin as the binder and it exhibited 8.71% activity recovery. This enzyme is a monomer with a molecular weight of 81.27 kDa, as estimated by SDS-PAGE. Optimum temperature and pH for cyclodextrin glycosyltransferase were 55°C and 8.0, respectively. The Michaelis-Menten constant was 8.62 g/l during maximum velocity of 0.858 g/l.h.en
dc.description.affiliationDepartment of Biochemistry and Microbiology Biological Sciences Institute UNESP-University Estadual Paulista, 1515 Bela Vista, Rio-Claro, SP
dc.description.affiliationChemical Engineering Department UEM-University Estadual de Maringá, Maringá
dc.description.affiliationDepartment of Food and Nutrition Faculty of Pharmaceutical Sciences UNESP-University Estadual Paulista, Araraquara
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology Biological Sciences Institute UNESP-University Estadual Paulista, 1515 Bela Vista, Rio-Claro, SP
dc.description.affiliationUnespDepartment of Food and Nutrition Faculty of Pharmaceutical Sciences UNESP-University Estadual Paulista, Araraquara
dc.format.extent48-53
dc.identifier.citationCzech Journal of Food Sciences, v. 32, n. 1, p. 48-53, 2014.
dc.identifier.issn1212-1800
dc.identifier.scopus2-s2.0-84894627774
dc.identifier.urihttp://hdl.handle.net/11449/171498
dc.language.isoeng
dc.relation.ispartofCzech Journal of Food Sciences
dc.relation.ispartofsjr0,355
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAffinity chromatography
dc.subjectCyclodextrin glycosyltransferase
dc.subjectPurification
dc.titleCyclodextrin production by bacillus lehensis isolated from cassava starch: Characterisation of a novel enzymeen
dc.typeArtigo

Arquivos