Cryopreservation of boar semen in 0.5mL straws at low spermatozoa concentration is better than high concentration to maintain sperm viability

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Ravagnani, Gisele M.
Torres, Mariana A.
Leal, Diego F.
Martins, Simone M. M. K.
Papa, Frederico O. [UNESP]
Dell'Aqua Junior, Jose A. [UNESP]
Alvarenga, Marco A. [UNESP]
Andrade, Andre F. C.

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Revista Pesquisa Veterinaria Brasileira


To date, no studies have been performed evaluating the effect of boar spermatozoa concentration in 0.5mL freezing straws, leading us to examine this question. Each sperm-rich fraction of the ejaculate (n=25) was diluted at five different sperm concentrations (100, 200, 300, 600 and 800 x 10(6) spermatozoa/mL), packaged in 0.5mL straws, and subsequently frozen. After thawing, the sperm from all of treatment groups were analyzed to determine motility characteristics using a sperm class analyzer (SCA-CASA), and their plasma and acrosomal membrane integrity, mitochondria! membrane potential, sperm membrane lipid peroxidation and fluidity were analyzed by flow cytometry. An increase in spermatozoa concentration above 300x10(6) spermatozoa/mL in a 0.5mL straw impaired (p<0.05) the total and progressive motility, curvilinear velocity, straight-line velocity, linearity and beat cross frequency. However, the plasma and acrosomal membrane integrity, mitochondria] membrane potential, membrane lipid peroxidation and fluidity were not influenced (p>0.05) by high spermatozoa concentrations at freezing. Therefore, to increase spermatozoa survival and total and progressive motility after thawing, boar spermatozoa should be frozen at concentrations up to 300x10(6) spermatozoa/mL.



Cryopreservation, boar semen, spermatozoa concentration, sperm viability, cryoinjury, cryocapacitation, swine

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Pesquisa Veterinaria Brasileira. Rio Janeiro: Revista Pesquisa Veterinaria Brasileira, v. 39, n. 9, p. 1726-1730, 2018.