Safety and efficacy of a Salmonella Gallinarum ΔcobSΔcbiA strain with potential to prevent chicken infections by Salmonella Gallinarum and Salmonella Enteritidis

dc.contributor.authorPaiva, J. B. [UNESP]
dc.contributor.authorPenha Filho, R. A C [UNESP]
dc.contributor.authorMoura, B. S. [UNESP]
dc.contributor.authorBerchieri, A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:26:26Z
dc.date.available2014-05-27T11:26:26Z
dc.date.issued2012-04-01
dc.description.abstractNew vaccine design techniques have allowed the development of effective vaccine strains against Salmonella infections in which the risks of reversion to the wild type and virulence is null. The mutant strain Salmonella Gallinarum ΔcobSΔcbiA was previously shown to be avirulent in chickens. In this study, this strain was tested as a vaccine against Salmonella Gallinarum (SG) and S. Enteritidis (SE) infections, and its protection levels, safety and possible risks of reversion to virulence after vaccination of layers were evaluated. Birds were vaccinated at five days of age or at five and 25 days of age. At 45 days of age, brown and white layers were challenged with SG and SE wild strains, respectively. Two assays to test the possibility of reversion to virulence were performed. Five successive bacterial passages in brown layers were carried out in the first assay. In the second assay, brown layers received a ten-fold concentrated inoculum of the SGΔcobSΔcbiA strain and were evaluated for clinical signs and mortality. In both experiments, no birds that received the inoculation of the attenuated strain died. Additionally, the use of the mutant strain as a vaccine provided good protection levels against both challenge strains.en
dc.description.affiliationDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV-Unesp)
dc.description.affiliationUnespDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal (FCAV-Unesp)
dc.format.extent115-120
dc.identifierhttp://dx.doi.org/10.1590/S1516-635X2012000200005
dc.identifier.citationRevista Brasileira de Ciencia Avicola, v. 14, n. 2, p. 115-120, 2012.
dc.identifier.doi10.1590/S1516-635X2012000200005
dc.identifier.file2-s2.0-84865356963.pdf
dc.identifier.issn1516-635X
dc.identifier.scieloS1516-635X2012000200005
dc.identifier.scopus2-s2.0-84865356963
dc.identifier.urihttp://hdl.handle.net/11449/73248
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Ciência Avícola
dc.relation.ispartofjcr0.463
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAttenuation
dc.subjectChicken
dc.subjectImmune response
dc.subjectReversion to virulence
dc.subjectSalmonella vaccine
dc.titleSafety and efficacy of a Salmonella Gallinarum ΔcobSΔcbiA strain with potential to prevent chicken infections by Salmonella Gallinarum and Salmonella Enteritidisen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/rbca/iaboutj.htm#03
unesp.author.lattes3508096260678286[4]
unesp.author.orcid0000-0003-2522-6500[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentPatologia Veterinária - FCAVpt

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