Anti-inflammatory activity of Alchornea triplinervia ethyl acetate fraction: Inhibition of H2O2, NO and TNF-alpha

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dc.contributor.authorMascia Lopes, Flavia Cristine [UNESP]
dc.contributor.authorCalvo, Tamara Regina [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorCarlos, Iracilda Zeppone [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:21:14Z
dc.date.available2014-05-20T14:21:14Z
dc.date.issued2010-12-01
dc.description.abstractContext: Alchornea triplinervia (Spreng.) Mull. Arg. (Euphorbiaceae) is a tree widespread in many Brazilian states. This plant naturally occurs in different ecosystems including tropical Atlantic forest, Amazon rain forest, moist tropical mixed forest, savanna forest, among others. Local populations traditionally use it in tea form to treat gastric disturbances.Objective: The objective of this research was to evaluate the plant A. triplinervia as a potential inhibitor of some macrophage functions involved in the inflammatory process.Materials and methods: The effects of Alchornea triplinervia ethyl acetate fraction (AtF) on hydrogen peroxide (H2O2), nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha) production in peritoneal macrophages were investigated using phenol red, Griess reagent and a sandwich immunoassay, respectively.Results: AtF chromatographic analyses indicate the presence of flavonoids as majority compounds. The fraction also showed an intense inhibition of H2O2 and NO production. The inhibitory effects of the fraction in H2O2 and NO production ranged from 72.25 +/- 4.68 to 69.64 +/- 4.21 and from 47.8 +/- 8.96 to 76.77 +/- 8.11%, respectively in the two tested concentrations, 15.62 and 62.5 mu g/mL. TNF-alpha production was partially inhibited in the tested concentrations and the inhibitory rate was around 18%.Discussion and conclusion: It is supposed that the elevated biological potential of A. triplinervia is related to the presence of phenolic compounds in the plant leaves. According to the results observed in this study, it is suggested that AtF presents anti-inflammatory activity, supporting the traditional use of A. triplinervia in Brazilian folk medicine.en
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Araraquara Inst Chem, Dept Organ Chem, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Clin Anal, Fac Pharmaceut Sci Araraquara, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Araraquara Inst Chem, Dept Organ Chem, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent1320-1327
dc.identifierhttp://dx.doi.org/10.3109/13880201003747463
dc.identifier.citationPharmaceutical Biology. London: Informa Healthcare, v. 48, n. 12, p. 1320-1327, 2010.
dc.identifier.doi10.3109/13880201003747463
dc.identifier.issn1388-0209
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/26354
dc.identifier.wosWOS:000284474500002
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofPharmaceutical Biology
dc.relation.ispartofjcr1.918
dc.relation.ispartofsjr0,563
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBiological activitiesen
dc.subjectimmune systemen
dc.subjectinflammationen
dc.subjectmedicinal planten
dc.titleAnti-inflammatory activity of Alchornea triplinervia ethyl acetate fraction: Inhibition of H2O2, NO and TNF-alphaen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dcterms.rightsHolderInforma Healthcare
unesp.author.orcid0000-0003-3032-2556[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt
unesp.departmentAnálises Clínicas - FCFpt

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Araraquara - FCF - Faculdade de Ciências Farmacêuticas
Artigos - Química Orgânica - IQ