Protective effects of spray-dried açaí (Euterpe oleracea Mart) fruit pulp against initiation step of colon carcinogenesis

dc.contributor.authorRomualdo, Guilherme Ribeiro [UNESP]
dc.contributor.authorFragoso, Mariana Franco [UNESP]
dc.contributor.authorBorguini, Renata Galhardo
dc.contributor.authorde Araújo Santiago, Manuela Cristina Pessanha
dc.contributor.authorFernandes, Ana Angélica Henrique [UNESP]
dc.contributor.authorBarbisan, Luis Fernando [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2018-12-11T16:59:25Z
dc.date.available2018-12-11T16:59:25Z
dc.date.issued2015-11-01
dc.description.abstractThe present study aims to evaluate whether açaí (Euterpe oleracea Mart) fruit pulp powder (AP) feeding attenuates the initiation step of chemically-induced mouse colon carcinogenesis. Male Swiss mice were fed low fat diet containing 2.5% or 5.0% of AP (weeks 1 to 4) produced by a spray-drying system. All groups received an intraperitoneal (i.p.) injection of colon carcinogen azoxymethane (AOM, 15 mg/kg of body weight) at week 3. After the first administration of carcinogen, blood samples were collected to perform Single Cell Gel Electrophoresis Assay. Some mice were euthanized at week 3 (n = 5 mice/group) and liver samples were collected for immunohistochemical and glutathione analysis. Othermice received a second i.p. injection of AOM at week 4 and were fed a high-fat diet to accelerate the development of preneoplastic aberrant crypt foci (ACF) until week 14 (n = 10 mice/group). Cyanidin 3-glucoside and cyanidin 3-rutinoside, lutein, a-carotene and ß-carotene were identified as the main anthocyanins and carotenoids in AP, respectively. At week 3, both dietary AP interventions (2.5% or 5.0%) reduced (p < 0.001) peripheral blood cell DNA damage induced by AOM. Moreover, dietary 5.0% AP increased (p = 0.002) hepatic total glutathione. At week 14, 5.0% AP intake reduced (p < 0.05) ACFmultiplicity. The findings indicate that AP feeding attenuates chemically-induced mouse colon carcinogenesis by increasing total GSH and attenuating DNA damage and preneoplastic lesion development.en
dc.description.affiliationUNESP - Univ. Estadual Paulista Botucatu Medical School Department of Pathology
dc.description.affiliationEmbrapa Food Technology
dc.description.affiliationUNESP - Univ. Estadual Paulista Biosciences Institute Department of Chemistry and Biochemistry, Botucatu, SP
dc.description.affiliationUNESP - Univ. Estadual Paulista Biosciences Institute Department of Morphology
dc.description.affiliationUnespUNESP - Univ. Estadual Paulista Botucatu Medical School Department of Pathology
dc.description.affiliationUnespUNESP - Univ. Estadual Paulista Biosciences Institute Department of Chemistry and Biochemistry, Botucatu, SP
dc.description.affiliationUnespUNESP - Univ. Estadual Paulista Biosciences Institute Department of Morphology
dc.format.extent432-440
dc.identifierhttp://dx.doi.org/10.1016/j.foodres.2015.08.037
dc.identifier.citationFood Research International, v. 77, p. 432-440.
dc.identifier.doi10.1016/j.foodres.2015.08.037
dc.identifier.file2-s2.0-84949087010.pdf
dc.identifier.issn0963-9969
dc.identifier.lattes3278528112652257
dc.identifier.scopus2-s2.0-84949087010
dc.identifier.urihttp://hdl.handle.net/11449/172261
dc.language.isoeng
dc.relation.ispartofFood Research International
dc.relation.ispartofsjr1,472
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAzoxymethane
dc.subjectAçaí (Euterpe oleracea Mart.)
dc.subjectColon carcinogenesis
dc.subjectDNA damage
dc.subjectMale Swiss mice
dc.titleProtective effects of spray-dried açaí (Euterpe oleracea Mart) fruit pulp against initiation step of colon carcinogenesisen
dc.typeArtigo
unesp.advisor.lattes3278528112652257
unesp.author.lattes0077247086732148[5]
unesp.author.orcid0000-0002-2180-1814[6]

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