Timing of prostaglandin F2α treatment in an estrogen-based protocol for timed artificial insemination or timed embryo transfer in lactating dairy cows

dc.contributor.authorPereira, M. H C [UNESP]
dc.contributor.authorSanches, C. P. [UNESP]
dc.contributor.authorGuida, T. G. [UNESP]
dc.contributor.authorRodrigues, A. D P [UNESP]
dc.contributor.authorAragon, F. L.
dc.contributor.authorVeras, M. B.
dc.contributor.authorBorges, P. T.
dc.contributor.authorWiltbank, M. C.
dc.contributor.authorVasconcelos, José Luiz Moraes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionPioneiros Veterinary Clinic
dc.contributor.institutionUniversity of Wisconsin
dc.date.accessioned2014-05-27T11:29:05Z
dc.date.available2014-05-27T11:29:05Z
dc.date.issued2013-05-01
dc.description.abstractObjectives were to investigate progesterone concentrations and fertility comparing 2 different intervals from PGF2α treatment and induced ovulation in an estrogen-based ovulation synchronization protocol for timed artificial insemination (TAI) or timed embryo transfer (TET) in lactating dairy cows. A total of 1,058 lactating Holstein cows [primiparous (n=371) and multiparous (n=687)], yielding 34.1±0.33 kg of milk/d at various days in milk were randomly assigned to receive treatment with PGF2α on either d 7 or 8 of the following protocol: d 0: 2mg of estradiol benzoate + controlled internal drug release device; d 8: controlled internal drug release device removal + 1.0mg of estradiol cypionate; d 10: TAI or d 17: TET. Only cows with a corpus luteum at d 17 received an embryo and all cows received GnRH at TET. Pregnancy diagnoses were performed by detection (transrectal ultrasonography) of an embryo on d 28 or a fetus on d 60. Fertility [pregnancy per artificial insemination (P/AI) or pregnancy per embryo transfer (P/ET)] was affected by breeding technique (AI vs. ET) and time of PGF2α treatment (d 7 vs. 8) at the 28-d pregnancy diagnosis for TAI [32.9% (238) vs. 20.6% (168)] and TET cows [47% (243) vs. 40.7% (244)] and at the 60-d pregnancy diagnosis for TAI [30% (238) vs. 19.2% (168)] and TET cows [37.9% (243) vs. 33.5% (244)]. The progesterone (P4) concentration at d 10 altered fertility in TAI cows, with higher P/AI in cows with P4 concentration <0.1 ng/mL compared with cows with P4 concentration ≥0.1 ng/mL, and in ET cows, with higher P/ET in cows with P4 concentration <0.22 ng/mL compared with cows with P4 concentration ≥0.22 ng/mL. Prostaglandin F2α treatment at d 7 increased the percentage of cows with P4 <0.1 ng/mL on d 10 [39.4 (85) vs. 23.2 (54)]. Reducing the period between PGF2α and TAI from 72 to 48h in dairy cows resulted in a clear reduction in fertility in cows bred by TAI and a subtle negative effect in cows that received TET. The earlier PGF2α treatment benefits are most likely mediated through gamete transport, fertilization, or early embryo development and a more subtle effect of earlier PGF2α treatment that may be mediated through changes in the uterine or hormonal environment that manifests itself after ET on d 7. © 2013 American Dairy Science Association.en
dc.description.affiliationDepartment of Animal Production São Paulo State University, Botucatu 18168-000
dc.description.affiliationPioneiros Veterinary Clinic, Carambei-PR 84145-000
dc.description.affiliationDepartment of Dairy Science University of Wisconsin, Madison 53706
dc.description.affiliationUnespDepartment of Animal Production São Paulo State University, Botucatu 18168-000
dc.format.extent2837-2846
dc.identifierhttp://dx.doi.org/10.3168/jds.2012-5840
dc.identifier.citationJournal of Dairy Science, v. 96, n. 5, p. 2837-2846, 2013.
dc.identifier.doi10.3168/jds.2012-5840
dc.identifier.issn0022-0302
dc.identifier.issn1525-3198
dc.identifier.lattes1069922096621313
dc.identifier.scopus2-s2.0-84876711681
dc.identifier.urihttp://hdl.handle.net/11449/75309
dc.identifier.wosWOS:000317703000013
dc.language.isoeng
dc.relation.ispartofJournal of Dairy Science
dc.relation.ispartofjcr2.749
dc.relation.ispartofsjr1,350
dc.relation.ispartofsjr1,350
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectDairy cow
dc.subjectFertility
dc.subjectProgesterone
dc.subjectProstaglandin F2α
dc.subjectBos
dc.titleTiming of prostaglandin F2α treatment in an estrogen-based protocol for timed artificial insemination or timed embryo transfer in lactating dairy cowsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes1069922096621313
unesp.author.orcid0000-0001-6324-3614[9]
unesp.author.orcid0000-0001-8188-0991[8]
unesp.author.orcid0000-0001-6620-3036[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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