Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs

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Instituto Oswaldo Cruz, Ministério da Saúde


Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.



trans-splicing, Trypanosoma cruzi, NFOH-121 (hydroxymethylnitrofurazone)

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Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 6, p. 617-620, 2004.