Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Nenhuma Miniatura disponível

Data

2017-10-01

Orientador

Coorientador

Pós-graduação

Curso de graduação

Título da Revista

ISSN da Revista

Título de Volume

Editor

Wiley-Blackwell

Tipo

Artigo

Direito de acesso

Acesso restrito

Resumo

Triploidization is an interesting tool to produce sterile fish. In the yellowtail tetra, Astyanax altiparanae, this can be applied for aquaculture and surrogate technologies. In this study, we compared the efficacy of cold (2 C) or heat shock (38 C, 40 C, and 42 C) on triploid induction in the yellowtail tetra. The eggs were treated with cold or heat shock, 2 min postfertilization (30min in cold shock or 2min in heat shock). Intact embryos served as the control group. Ploidy status was confirmed by karyotyping, flow cytometry, and nuclear diameter of erythrocytes. The hatching rate decreased after cold shock (12.69 +/- 15.76%) and heat shock at 42 C(0.35 +/- 0.69%) in comparison with the control group (63.19 +/- 16.82%). At 38 C and 40 C, hatching rates (61.29 +/- 17.73% and 61.75 +/- 22.1%, respectively) were not decreased. Only one triploid arose at 38 C (1/80). At 40 C, a high number of triploids arose (72/78). At 42 C, very few embryos developed into the hatching stage. A large number of haploid individuals arose after cold shock (61/75), with only one triploid. Our results indicate that heat shocking of embryos at 40 C is optimum for triploid production in the yellowtail tetra.

Descrição

Palavras-chave

Idioma

Inglês

Como citar

Journal Of The World Aquaculture Society. Hoboken: Wiley, v. 48, n. 5, p. 741-750, 2017.

Itens relacionados