Effectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Study

dc.contributor.authorCavalli, Daiana [UNESP]
dc.contributor.authorToia, Cássia Cestari [UNESP]
dc.contributor.authorFlores Orozco, Esteban Isai [UNESP]
dc.contributor.authorKhoury, Rayana Duarte [UNESP]
dc.contributor.authorCardoso, Flávia Goulart da Rosa [UNESP]
dc.contributor.authorAlves, Marcelo Corrêa
dc.contributor.authorCarvalho, Cláudio Antônio Talge [UNESP]
dc.contributor.authorValera, Marcia Carneiro [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:12:37Z
dc.date.available2018-12-11T17:12:37Z
dc.date.issued2017-08-01
dc.description.abstractIntroduction This clinical study was conducted to correlate the microbiological profile and levels of endotoxins found in primary endodontic infection with the presence of clinical features and to evaluate the removal of microorganisms and endotoxins using rotary, reciprocating, and hybrid systems for biomechanical preparation. Methods Thirty single root canals with primary endodontic infection were evaluated with signs and symptoms and were randomly divided into 3 groups according to the instrumentation system used (n = 10) as follows: rotary Mtwo instruments (VDW, Munich, Germany) with 8 files, the reciprocating Reciproc system (VDW) with a single file, and Genius hybrid instruments with 3 files (1 rotary and 2 reciprocating files) with irrigation using 24 mL 2.5% sodium hypochlorite. Samples were collected before (S1) and after instrumentation (S2) before being submitted to microbiological culture (colony-forming units/mL) and the checkerboard DNA-DNA hybridization test. Endotoxins were quantified using the limulus amebocyte lysate assay. Results Microbiological culture showed statistical differences in the reduction of colony-forming units/mL with all systems tested (P <.05), but no statistical difference was found among the groups. The most frequently detected species were Capnocytophaga ochracea (53%) and Fusobacterium nucleatum (53%) at S1 and F. nucleatum (50%) and Leptotrichia buccalis (50%) at S2. As for the reduction of endotoxins at S2, Mtwo presented the best results (95.05%) followed by the Genius (91.85%) and Reciproc (64.68%) groups, but no statistical difference was found among the groups. Previous pain, tenderness to percussion, and presence of a sinus tract were associated with specific microorganisms (P <.05). Conclusions Signs and symptoms were correlated with microorganisms. Endodontic treatment was effective in reducing bacteria and endotoxins but was not capable of completely removing them from the root canal.en
dc.description.affiliationDepartment of Restorative Dentistry Endodontic Division São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos
dc.description.affiliationSuperior School of Agriculture “Luiz de Queiroz” Technical Section of Information Technology University of São Paulo
dc.description.affiliationUnespDepartment of Restorative Dentistry Endodontic Division São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos
dc.format.extent1237-1245
dc.identifierhttp://dx.doi.org/10.1016/j.joen.2017.03.032
dc.identifier.citationJournal of Endodontics, v. 43, n. 8, p. 1237-1245, 2017.
dc.identifier.doi10.1016/j.joen.2017.03.032
dc.identifier.file2-s2.0-85020414336.pdf
dc.identifier.issn0099-2399
dc.identifier.lattes9304600166583100
dc.identifier.orcid0000-0003-0987-5594
dc.identifier.scopus2-s2.0-85020414336
dc.identifier.urihttp://hdl.handle.net/11449/174734
dc.language.isoeng
dc.relation.ispartofJournal of Endodontics
dc.relation.ispartofsjr1,585
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCheckerboard DNA-DNA hybridization
dc.subjectinstrumentation
dc.subjectprimary endodontic infection
dc.titleEffectiveness in the Removal of Endotoxins and Microbiological Profile in Primary Endodontic Infections Using 3 Different Instrumentation Systems: A Randomized Clinical Studyen
dc.typeArtigo
unesp.author.lattes9304600166583100[7]
unesp.author.orcid0000-0003-0987-5594[7]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Carregando...
Imagem de Miniatura
Nome:
2-s2.0-85020414336.pdf
Tamanho:
1.23 MB
Formato:
Adobe Portable Document Format
Descrição: