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RAPD marker use for improving resistance to Helicoverpa zea in corn

dc.contributor.authorda Silva, S. C.
dc.contributor.authorLemos, MVF
dc.contributor.authorOsuna, JTA
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:13:02Z
dc.date.available2014-05-20T13:13:02Z
dc.date.issued2000-01-01
dc.description.abstractHelicoverpa zea is responsible for great losses to the corn, Zen mays L., crops final productivity, and the best way to control it is by improving genetic resistance. In collaboration with corn improvement and increasing resistance to insects through molecular marker assisted selection, this work had as an objective the selection of resistant (RP) and susceptible progenies (SP) to H. zea based on the RAPD technique. Molecular markers were Found, among the resistant progenies and it is suggested that linkage of these within the Zapalote Chico corn race, be used to extract resistance genes from this race as a donor. The progenies were selected from a population of half-sibs exhibiting a broader genetic base (FCAVJ-VF14). After DNA extraction, two sample bulks were formed; one made up of the six most resistant plants, the other of the six least resistant plants. Eighty-six primers were tested for PCR reactions with the resistant and susceptible bulks and analyzed on agarose electrophoresis for the detection of RAPD band polymorphism. The results of the banding patterns and similarity values indicated a nucleotide sequence amplified by the primer OPC-2 as a possible molecular marker for the identification of resistant progenies and a homology region between them and the Zapalote Chico corn race.en
dc.description.affiliationUNESP, Dept Biol Aplicada Agropecuaria, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUSP, FMRP, Dept Genet, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol Aplicada Agropecuaria, BR-14884900 Jaboticabal, SP, Brazil
dc.format.extent289-294
dc.identifierhttp://www.maydica.org/back_issues.html
dc.identifier.citationMaydica. Bergamo: Maydica-ist Sper Cerealicoltur, v. 45, n. 4, p. 289-294, 2000.
dc.identifier.issn0025-6153
dc.identifier.lattes7179273060624761
dc.identifier.urihttp://hdl.handle.net/11449/945
dc.identifier.wosWOS:000167030600003
dc.language.isoeng
dc.publisherMaydica-ist Sper Cerealicoltur
dc.relation.ispartofMaydica
dc.relation.ispartofjcr0.231
dc.relation.ispartofsjr0,263
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectZea mayspt
dc.subjectRAPDpt
dc.subjectmarker-assisted selectionpt
dc.titleRAPD marker use for improving resistance to Helicoverpa zea in cornen
dc.typeArtigo
dcterms.licensehttp://www.maydica.org/about_maydica.html
dcterms.rightsHolderMaydica-ist Sper Cerealicoltur
unesp.author.lattes7179273060624761
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentBiologia - FCAVpt

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