Laser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficit

dc.contributor.authorda Silva, Paula Corrêa Silveira
dc.contributor.authorMarques, Nelson Pereira
dc.contributor.authorFarina, Marcella Tassi
dc.contributor.authorOliveira, Thais Marchini
dc.contributor.authorDuque, Cristiane [UNESP]
dc.contributor.authorMarques, Nádia Carolina Teixeira
dc.contributor.authorSakai, Vivien Thiemy
dc.contributor.institutionFederal University of Alfenas (UNIFAL-MG)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionJosé do Rosário Vellano University (UNIFENAS)
dc.date.accessioned2018-12-11T16:54:19Z
dc.date.available2018-12-11T16:54:19Z
dc.date.issued2018-07-06
dc.description.abstractThis study aimed to analyze the effects of laser irradiation on the membrane integrity and viability of stem cells from human exfoliated deciduous teeth (SHED) that were kept in serum starvation. Nutritional deficit was used to mimic the cellular stress conditions of SHED isolation for regenerative dental approaches, where laser therapy could be beneficial. SHED were cultured under serum starvation (MEMα + 1%FBS) for 1 or 24 h pre-irradiation (protocols A and B, respectively). Then, cells received low-level laser therapy (LLLT; 660 nm) at 2.5 J/cm2 (0.10 W; groups I and V), 5.0 J/cm2 (0.20 W; groups II and VI), 7.5 J/cm2 (0.30 W; groups III and VII), or remained non-irradiated (groups IV and VIII). During irradiation, cells were maintained in 1% FBS (groups I–IV) or 10% FBS (normal culture conditions; groups V–VIII). Membrane integrity was evaluated by quantifying lactate dehydrogenase (LDH) release (immediately after irradiation), and cell viability was assessed by the MTT assay (24, 48, and 72 h post-irradiation). Serum starvation did not alter LDH release by non-irradiated SHED, while LDH release decreased significantly in groups irradiated in 1% FBS (I and III), but not in groups irradiated in 10% FBS (V–VII), regardless the pre-irradiation conditions (protocols A/B). Cell viability was significantly higher 24 h after irradiation, in most protocol A groups. In contrast, cell viability remained mostly unaltered in protocol B groups. LLLT contributed to maintain membrane integrity in SHED subjected to nutritional deficit before and during irradiation with 0.10 or 0.30 W. Short serum starvation before irradiation improved SHED viability at 24 h post-irradiation.en
dc.description.affiliationDepartment of Clinics and Surgery School of Dentistry Federal University of Alfenas (UNIFAL-MG), Rua Gabriel Monteiro da Silva, 700.
dc.description.affiliationDepartment of Pediatric Dentistry Orthodontics and Public Health Bauru School of Dentistry University of São Paulo (USP)
dc.description.affiliationDepartment of Pediatric Dentistry and Public Health School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Pediatric Dentistry School of Dentistry José do Rosário Vellano University (UNIFENAS)
dc.description.affiliationUnespDepartment of Pediatric Dentistry and Public Health School of Dentistry São Paulo State University (UNESP)
dc.format.extent1-7
dc.identifierhttp://dx.doi.org/10.1007/s10103-018-2574-x
dc.identifier.citationLasers in Medical Science, p. 1-7.
dc.identifier.doi10.1007/s10103-018-2574-x
dc.identifier.file2-s2.0-85049587946.pdf
dc.identifier.issn1435-604X
dc.identifier.issn0268-8921
dc.identifier.lattes5651874509493617
dc.identifier.orcid0000-0002-2575-279X
dc.identifier.scopus2-s2.0-85049587946
dc.identifier.urihttp://hdl.handle.net/11449/171190
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCell culture techniques
dc.subjectCell survival
dc.subjectL-lactate dehydrogenase
dc.subjectLow-level light therapy
dc.subjectStem cells
dc.titleLaser treatment contributes to maintain membrane integrity in stem cells from human exfoliated deciduous teeth (shed) under nutritional deficiten
dc.typeArtigo
unesp.author.lattes5651874509493617[5]
unesp.author.orcid0000-0002-5574-4539[7]
unesp.author.orcid0000-0002-2575-279X[5]

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