Optimized architecture for Tyrosinase-containing Langmuir-Blodgett films to detect pyrogallol

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dc.contributor.authorPavinatto, F. J.
dc.contributor.authorFernandes, E. G. R.
dc.contributor.authorAlessio, P. [UNESP]
dc.contributor.authorConstantino, C. J. L. [UNESP]
dc.contributor.authorde Saja, J. A.
dc.contributor.authorZucolotto, V.
dc.contributor.authorApetrei, C.
dc.contributor.authorOliveira, O. N.
dc.contributor.authorRodriguez-Mendez, M. L.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Valladolid
dc.contributor.institutionDunarea de Jos Univ Galati
dc.date.accessioned2014-05-20T13:23:05Z
dc.date.available2014-05-20T13:23:05Z
dc.date.issued2011-01-01
dc.description.abstractThe control of molecular architectures has been a key factor for the use of Langmuir-Blodgett (LB) films in biosensors, especially because biomolecules can be immobilized with preserved activity. In this paper we investigated the incorporation of tyrosinase (Tyr) in mixed Langmuir films of arachidic acid (AA) and a lutetium bisphthalocyanine (LuPc2), which is confirmed by a large expansion in the surface pressure isotherm. These mixed films of AA-LuPc2 + Tyr could be transferred onto ITO and Pt electrodes as indicated by FTIR and electrochemical measurements, and there was no need for crosslinking of the enzyme molecules to preserve their activity. Significantly, the activity of the immobilised Tyr was considerably higher than in previous work in the literature, which allowed Tyr-containing LB films to be used as highly sensitive voltammetric sensors to detect pyrogallol. Linear responses have been found up to 400 mu M, with a detection limit of 4.87 x 10(-2) mu M (n = 4) and a sensitivity of 1.54 mu A mu M-1 cm(-2). In addition, the Hill coefficient (h = 1.27) indicates cooperation with LuPc2 that also acts as a catalyst. The enhanced performance of the LB-based biosensor resulted therefore from a preserved activity of Tyr combined with the catalytic activity of LuPc2, in a strategy that can be extended to other enzymes and analytes upon varying the LB film architecture.en
dc.description.affiliationUniv São Paulo, Inst Fis São Carlos, Grp Polimeros Bernhard Gross, BR-13566590 São Carlos, SP, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil
dc.description.affiliationUniv Valladolid, ETS Ingenieros Ind, E-47011 Valladolid, Spain
dc.description.affiliationUniv Valladolid, Fac Ciencias, E-47011 Valladolid, Spain
dc.description.affiliationDunarea de Jos Univ Galati, Fac Sci, Dept Chem, Galati 800008, Romania
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Fac Ciencias & Tecnol, BR-19060900 Presidente Prudente, SP, Brazil
dc.format.extent4995-5003
dc.identifierhttp://dx.doi.org/10.1039/c0jm03864d
dc.identifier.citationJournal of Materials Chemistry. Cambridge: Royal Soc Chemistry, v. 21, n. 13, p. 4995-5003, 2011.
dc.identifier.doi10.1039/c0jm03864d
dc.identifier.issn0959-9428
dc.identifier.lattes9727122203219263
dc.identifier.orcid0000-0002-1345-0540
dc.identifier.urihttp://hdl.handle.net/11449/6908
dc.identifier.wosWOS:000288450200036
dc.language.isoeng
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofJournal of Materials Chemistry
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleOptimized architecture for Tyrosinase-containing Langmuir-Blodgett films to detect pyrogallolen
dc.typeArtigo
dcterms.licensehttp://www.rsc.org/Publishing/librarians/AuthorDeposition.asp
dcterms.rightsHolderRoyal Soc Chemistry
unesp.author.lattes9727122203219263[3]
unesp.author.lattes6118325967319836[4]
unesp.author.orcid0000-0001-7252-2949[5]
unesp.author.orcid0000-0003-4307-3077[6]
unesp.author.orcid0000-0002-3823-4174[7]
unesp.author.orcid0000-0002-1345-0540[3]
unesp.author.orcid0000-0002-9760-362X[9]
unesp.author.orcid0000-0002-5399-5860[8]
unesp.author.orcid0000-0002-5921-3161[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept
unesp.departmentFísica, Química e Biologia - FCTpt

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