The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin
dc.contributor.author | da Silva Neto, Benedito Rodrigues | |
dc.contributor.author | da Silva, Julhiany de Fatima [UNESP] | |
dc.contributor.author | Mendes-Giannini, Maria José Soares [UNESP] | |
dc.contributor.author | Lenzi, Henrique Leonel | |
dc.contributor.author | de Almeida Soares, Celia Maria | |
dc.contributor.author | Pereira, Maristela | |
dc.contributor.institution | Universidade Federal de Goiás (UFG) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Fiocruz MS | |
dc.date.accessioned | 2014-05-20T13:23:50Z | |
dc.date.available | 2014-05-20T13:23:50Z | |
dc.date.issued | 2009-12-24 | |
dc.description.abstract | Background: The pathogenic fungus Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis (PCM). This is a pulmonary mycosis acquired by inhalation of fungal airborne propagules that can disseminate to several organs and tissues leading to a severe form of the disease. Adhesion and invasion to host cells are essential steps involved in the internalization and dissemination of pathogens. Inside the host, P. brasiliensis may use the glyoxylate cycle for intracellular survival.Results: Here, we provide evidence that the malate synthase of P. brasiliensis (PbMLS) is located on the fungal cell surface, and is secreted. PbMLS was overexpressed in Escherichia coli, and polyclonal antibody was obtained against this protein. By using Confocal Laser Scanning Microscopy, PbMLS was detected in the cytoplasm and in the cell wall of the mother, but mainly of budding cells of the P. brasiliensis yeast phase. PbMLSr and its respective polyclonal antibody produced against this protein inhibited the interaction of P. brasiliensis with in vitro cultured epithelial cells A549.Conclusion: These observations indicated that cell wall-associated PbMLS could be mediating the binding of fungal cells to the host, thus contributing to the adhesion of fungus to host tissues and to the dissemination of infection, behaving as an anchorless adhesin. | en |
dc.description.affiliation | Univ Fed Goias, Inst Ciencias Biol, Dept Bioquim & Biol Mol, Mol Biol Lab, BR-74001970 Goiania, Go, Brazil | |
dc.description.affiliation | UNESP, Univ Estadual Paulista, Lab Micol Clin, Araraquara, SP, Brazil | |
dc.description.affiliation | Fiocruz MS, Inst Oswaldo Cruz, Lab Patol, BR-21045900 Rio de Janeiro, Brazil | |
dc.description.affiliationUnesp | UNESP, Univ Estadual Paulista, Lab Micol Clin, Araraquara, SP, Brazil | |
dc.description.sponsorship | Ministerio de Ciência e Tecnologia (MCT) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Financiadora de Estudos e Projetos (FINEP) | |
dc.description.sponsorship | International Foundation for Science (IFS), Stockholm, Sweden | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.format.extent | 12 | |
dc.identifier | http://dx.doi.org/10.1186/1471-2180-9-272 | |
dc.identifier.citation | Bmc Microbiology. London: Biomed Central Ltd., v. 9, p. 12, 2009. | |
dc.identifier.doi | 10.1186/1471-2180-9-272 | |
dc.identifier.file | WOS000273911100001.pdf | |
dc.identifier.issn | 1471-2180 | |
dc.identifier.orcid | 0000-0002-8059-0826 | |
dc.identifier.uri | http://hdl.handle.net/11449/7252 | |
dc.identifier.wos | WOS:000273911100001 | |
dc.language.iso | eng | |
dc.publisher | Biomed Central Ltd. | |
dc.relation.ispartof | BMC Microbiology | |
dc.relation.ispartofjcr | 2.829 | |
dc.relation.ispartofsjr | 1,242 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.title | The malate synthase of Paracoccidioides brasiliensis is a linked surface protein that behaves as an anchorless adhesin | en |
dc.type | Artigo | |
dcterms.license | http://www.biomedcentral.com/about/license | |
dcterms.rightsHolder | Biomed Central Ltd. | |
unesp.author.orcid | 0000-0002-8059-0826[3] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquara | pt |
unesp.department | Análises Clínicas - FCF | pt |
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