Incorporation of labeled methionine as a tissue tracer in broiler chickens

dc.contributor.authorStradiotti, Ana Cristina [UNESP]
dc.contributor.authorBendassolli, J. A.
dc.contributor.authorDucatti, C. [UNESP]
dc.contributor.authorSartori, J. R. [UNESP]
dc.contributor.authorPelícia, V. C. [UNESP]
dc.contributor.authorAraujo, P. C. [UNESP]
dc.contributor.authorMaruno, M. K. [UNESP]
dc.contributor.authorPezzato, A. C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:09:49Z
dc.date.available2018-12-11T17:09:49Z
dc.date.issued2016-10-01
dc.description.abstractThe objective of this study was to evaluate the process of L-methionine incorporation in the blood plasma, liver, breast muscle, and abdominal fat of 35- to 59-d-old broiler chickens using the carbon stable isotope (12C and 13C) technique for the estimation of methionine requirements. In this experiment, 51 male broiler chickens orally received a solution of L-[13C1] methionine (92 atm % 13C) at 29 µmol/kg live weight/h for 6 h. Three birds were sacrificed for tissue collection at times 0 h (control), 0.5, 1, 2, 3, 4, 5, 6, 12, 24, 48, 72, 96, 120, 144, 168, and 336 h after the administration of the first dose. Tissue L-[13C1] methionine incorporation mass and percentage results were analyzed using Minitab 16 statistical software. Except for abdominal fat, tissue methionine levels gradually increased after the administration of the methionine solution. The calculated half-lives of methionine in the blood plasma, liver, and breast muscle were 2.52, 1.36, and 3.57 h, respectively, suggesting a greater rate of methionine incorporation in the liver, followed by blood plasma and breast muscle. The isotopic dilution showed that 2.81, 4.79, and 23.64% of the administered L-methionine were retained in the blood plasma, liver, and breast muscle, respectively. The methionine requirements of finisher broilers may be estimated using the carbon isotope technique, and approximately 3, 5, and 24% methionine is used for the synthesis of blood plasma, liver, and breast muscle, respectively, at the evaluated dose.en
dc.description.affiliationFMVZ/UNESP
dc.description.affiliationCenter of Nuclear Energy in Agriculture CENA/USP
dc.description.affiliationDepartment of Physics and Biophysics IBB/UNESP
dc.description.affiliationDepartment of Genetic Improvement and Animal Nutrition FMVZ/UNESP
dc.description.affiliationUnespFMVZ/UNESP
dc.description.affiliationUnespDepartment of Physics and Biophysics IBB/UNESP
dc.description.affiliationUnespDepartment of Genetic Improvement and Animal Nutrition FMVZ/UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 07/57776-0
dc.format.extent719-724
dc.identifierhttp://dx.doi.org/10.1590/1516-635x1804719-724
dc.identifier.citationRevista Brasileira de Ciencia Avicola, v. 18, n. 4, p. 719-724, 2016.
dc.identifier.doi10.1590/1516-635x1804719-724
dc.identifier.fileS1516-635X2016000400719.pdf
dc.identifier.issn1516-635X
dc.identifier.scieloS1516-635X2016000400719
dc.identifier.scopus2-s2.0-85012195292
dc.identifier.urihttp://hdl.handle.net/11449/174201
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Ciencia Avicola
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectIsotopic dilution
dc.subjectLabeled isotopes
dc.subjectTurnover
dc.titleIncorporation of labeled methionine as a tissue tracer in broiler chickensen
dc.typeArtigo
unesp.author.lattes9229984315982663[8]
unesp.author.orcid0000-0001-9543-5688[8]

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