Chemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranes

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dc.contributor.authorRuiz, Gilia Cristine Marques [UNESP]
dc.contributor.authordo Carmo Morato, Luis Fernando [UNESP]
dc.contributor.authorPazin, Wallance Moreira [UNESP]
dc.contributor.authorMilano, Francesco
dc.contributor.authorConstantino, Carlos José Leopoldo [UNESP]
dc.contributor.authorValli, Ludovico
dc.contributor.authorGiotta, Livia
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionItalian National Research Council (CNR-ISPA)
dc.contributor.institutionS.P. Lecce-Monteroni
dc.contributor.institutionUnità di Lecce
dc.date.accessioned2021-06-25T11:15:08Z
dc.date.available2021-06-25T11:15:08Z
dc.date.issued2021-08-01
dc.description.abstractThe lack of studies involving the effects in human health associated with the chronic ingestion of pollutants lead to the path of investigating the action of these compounds in cell membrane models. We demonstrated the interaction (causes and consequences) of the hormone 17 α-ethinylestradiol (EE2) with lipid monolayers (prepared as Langmuir films) and bilayers prepared as small unilamellar vesicles (SUVs) and giant unilamellar vesicles (GUVs). Both fluidity and majority chemical composition of real plasma cell membrane were guaranteed using the phospholipid 1-palmitoil-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC). Surface pressure-mean molecular area (π-A) isotherms and PM-IRRAS measurements highlighted the strong interaction of EE2 with POPC monolayers, leading the hormone to remain at the air/water interface and promoting its penetration into the phospholipid hydrophobic chains. In the case of bilayers, the entrance of the hormone inside the SUV is likely facilitated by their high curvature. In GUVs, EE2 was responsible for changes in the spherical shape, forming structures like buds and lipid protrusions. The set of results indicates the strong effects of EE2 on fluid membranes, which is an important feature to predict its damage in human cells.en
dc.description.affiliationDepartment of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)
dc.description.affiliationInstitute of Sciences of Food Production Italian National Research Council (CNR-ISPA), S.P. Lecce-Monteroni
dc.description.affiliationDepartment of Biological and Environmental Sciences and Technologies (DiSTeBA) University of Salento S.P. Lecce-Monteroni
dc.description.affiliationConsorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM) Unità di Lecce, S.P. Lecce-Monteroni
dc.description.affiliationUnespDepartment of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipInstituto Nacional de Ciência e Tecnologia em Eletrônica Orgânica
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2016/09633-4
dc.description.sponsorshipIdFAPESP: 2018/222147-6
dc.description.sponsorshipIdCAPES: 88882.330129/2018-01
dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2021.111794
dc.identifier.citationColloids and Surfaces B: Biointerfaces, v. 204.
dc.identifier.doi10.1016/j.colsurfb.2021.111794
dc.identifier.issn1873-4367
dc.identifier.issn0927-7765
dc.identifier.scopus2-s2.0-85104909298
dc.identifier.urihttp://hdl.handle.net/11449/208618
dc.language.isoeng
dc.relation.ispartofColloids and Surfaces B: Biointerfaces
dc.sourceScopus
dc.subject17α-ethynylestradiol
dc.subjectGiant unilamellar vesicles
dc.subjectLangmuir monolayers
dc.subjectPM-IRRAS
dc.subjectPOPC
dc.subjectSmall unilamellar vesicles
dc.titleChemical and morphological effects of the contraceptive hormone 17 α-ethynylestradiol on fluid lipid membranesen
dc.typeArtigo
unesp.author.orcid0000-0002-2507-3738[2]
unesp.author.orcid0000-0002-2157-5933[3]

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