Proteome and phosphoproteome of Africanized and European honeybee venoms
Nenhuma Miniatura disponível
Data
2013-09-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
Honey bee venom toxins trigger immunological, physiological, and neurological responses within victims. The high occurrence of bee attacks involving potentially fatal toxic and allergic reactions in humans and the prospect of developing novel pharmaceuticals make honey bee venom an attractive target for proteomic studies. Using label-free quantification, we compared the proteome and phosphoproteome of the venom of Africanized honeybees with that of two European subspecies, namely Apis mellifera ligustica and A. m. carnica. From the total of 51 proteins, 42 were common to all three subspecies. Remarkably, the toxins melittin and icarapin were phosphorylated. In all venoms, icarapin was phosphorylated at the 205Ser residue, which is located in close proximity to its known antigenic site. Melittin, the major toxin of honeybee venoms, was phosphorylated in all venoms at the 10Thr and 18Ser residues. 18Ser phosphorylated melittin-the major of its two phosphorylated forms-was less toxic compared to the native peptide. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Descrição
Palavras-chave
Animal proteomics, Apis mellifera, Honeybee venom, LC-MS/MS, Melittin, Phosphorylation, amino acid, bee venom, icarapin, melittin, phosphoprotein, phosphoproteome, proteome, serine, unclassified drug, Africa, amino acid substitution, Europe, honeybee, insect, liquid chromatography, mass spectrometry, nonhuman, peptide analysis, priority journal, protein analysis, protein database, protein phosphorylation, proteomics, quantitative analysis, species comparison, toxin analysis
Idioma
Inglês
Como citar
Proteomics, v. 13, n. 17, p. 2638-2648, 2013.
