Dose-response of diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] in the urothelial mucosa of Wistar rats

dc.contributor.authorCardoso, Ana Paula Ferragut [UNESP]
dc.contributor.authorIhlaseh Catalano, Shadia Muhammad [UNESP]
dc.contributor.authorda Rocha, Mitscheli Sanches [UNESP]
dc.contributor.authorNascimento e Pontes, Merielen Garcia [UNESP]
dc.contributor.authorCamargo, João Lauro Viana de [UNESP]
dc.contributor.authorOliveira, Maria Luiza Cotrim Sartor de [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:11Z
dc.date.available2014-05-27T11:30:11Z
dc.date.issued2013-08-20
dc.description.abstractDiuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] is a herbicide that induced urothelial tumors in the urinary bladder of Wistar rats fed 2500. ppm during a long-term study. The currently suggested non-genotoxic mode of action (MOA) of diuron encompasses in succession urothelial necrosis induced by direct cytotoxicity, regenerative cell proliferation and sustained urothelial hyperplasia that increases the likelihood of neoplasia development. This study evaluated the dose-response profile of urothelial histological and ultrastructural lesions induced by diuron. Sixty male Wistar rats were fed ad libitum diuron mixed in the diet at 0, 60, 125, 500, 1250, or 2500. ppm for 20 weeks. The incidences of urothelial simple hyperplasia and the cell proliferation index were significantly increased in the diuron-fed 1250 and 2500. ppm groups. By scanning electron microscopy, the incidences and severity of lesions were significantly increased in the 500 and 1250. ppm groups. The incidences of urothelial hyperplasia in the kidney pelvis were significantly increased in the 500, 1250 and 2500. ppm groups. The present study documents the dose-response influence of diuron on the rat urothelium, with a no observed effect level (NOEL) at 125. ppm; 1250. ppm was as effective as 2500. ppm at inducing urothelial lesions. © 2013 Elsevier B.V.en
dc.description.affiliationCenter for the Evaluation of the Environmental Impact on Human Health (TOXICAM) Department of Pathology Botucatu Medical School, UNESP - São Paulo State University, Botucatu 18618-970, São Paulo
dc.description.affiliationUnespCenter for the Evaluation of the Environmental Impact on Human Health (TOXICAM) Department of Pathology Botucatu Medical School, UNESP - São Paulo State University, Botucatu 18618-970, São Paulo
dc.format.extent1-5
dc.identifierhttp://dx.doi.org/10.1016/j.tox.2013.07.007
dc.identifier.citationToxicology, v. 312, n. 1, p. 1-5, 2013.
dc.identifier.doi10.1016/j.tox.2013.07.007
dc.identifier.issn0300-483X
dc.identifier.issn1879-3185
dc.identifier.scopus2-s2.0-84881496794
dc.identifier.urihttp://hdl.handle.net/11449/76292
dc.identifier.wosWOS:000327005300001
dc.language.isoeng
dc.relation.ispartofToxicology
dc.relation.ispartofjcr3.265
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCell proliferation
dc.subjectCytotoxicity
dc.subjectDiuron
dc.subjectDose-response
dc.subjectRat urothelial hyperplasia
dc.subjectScanning electron microscopy
dc.subjectdiuron
dc.subjectanimal tissue
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectdiet
dc.subjectdose response
dc.subjecthistopathology
dc.subjecthyperplasia
dc.subjectkidney pelvis
dc.subjectmale
dc.subjectmucosa
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrat
dc.subjectscanning electron microscopy
dc.subjecturothelium
dc.subjectweight gain
dc.titleDose-response of diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] in the urothelial mucosa of Wistar ratsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.orcid0000-0002-8021-5006[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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