Simultaneous observation of collagen and elastin in normal and pathological tissues: analysis of Sirius-red-stained sections by fluorescence microscopy

dc.contributor.authorBorges, L. F.
dc.contributor.authorTaboga, SR
dc.contributor.authorGutierrez, P. S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:24:25Z
dc.date.available2014-05-20T15:24:25Z
dc.date.issued2005-06-01
dc.description.abstractIn order to observe collagen and elastic fibers simultaneously, sections of human aorta, skin, lung, liver, and bladder were stained by Sirius red and analyzed by fluorescence microscopy. In all cases, the fibers of collagen presented the characteristic fluorescent red-orange color that results from the interaction of this extracellular protein with the dye, whereas elastic fibers showed strong green fluorescence (intrinsic fluorescence). This method efficiently detects collagen and elastic fibers when these two structures are present and could have valuable applications in processes that involves both fibers.en
dc.description.affiliationUniv São Paulo, Sch Med, Heart Inst InCor, Pathol Lab, BR-05403000 São Paulo, Brazil
dc.description.affiliationUNESP, Dept Biol, Sao Jose do Rio Preto, Brazil
dc.description.affiliationUnespUNESP, Dept Biol, Sao Jose do Rio Preto, Brazil
dc.format.extent551-552
dc.identifierhttp://dx.doi.org/10.1007/s00441-005-1108-6
dc.identifier.citationCell and Tissue Research. New York: Springer, v. 320, n. 3, p. 551-552, 2005.
dc.identifier.doi10.1007/s00441-005-1108-6
dc.identifier.issn0302-766X
dc.identifier.lattes1445259468526188
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.urihttp://hdl.handle.net/11449/35035
dc.identifier.wosWOS:000229511300020
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofCell and Tissue Research
dc.relation.ispartofjcr3.043
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcollagenpt
dc.subjectelastinpt
dc.subjectSirius redpt
dc.subjectfluorescencept
dc.subjecthumanpt
dc.titleSimultaneous observation of collagen and elastin in normal and pathological tissues: analysis of Sirius-red-stained sections by fluorescence microscopyen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes1445259468526188
unesp.author.orcid0000-0002-0970-4288[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição: