Rapid turbidimetric assay to determine the potency of daptomycin in lyophilized powder

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dc.contributor.authorTótoli, Eliane Gandolpho [UNESP]
dc.contributor.authorSalgado, Hérida Regina Nunes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:36:02Z
dc.date.available2015-12-07T15:36:02Z
dc.date.issued2015
dc.description.abstractDaptomycin is an important antimicrobial for clinical practice, mainly because it remains very active against Gram-positive resistant strains, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci. Development of microbiological methods for the analysis of antimicrobials is highly recommended, since they can provide important information about their biological activities, which physicochemical methods are not able to provide. Considering that there are no studies in the literature describing microbiological methods for the analysis of daptomycin, the aim of this work was to validate a microbiological method for the quantitation of daptomycin by the turbidimetric assay. Staphylococcus aureus was used as the test microorganism, and the brain heart infusion broth was used as the culture medium. The validation of the method was performed according to the ICH guidelines, and it was shown to be linear, precise, robust, accurate and selective, over a concentration range of 8.0 to 18.0 µg mL-1. Student's t-test showed the interchangeability of the proposed method with a previously-validated HPLC method. The developed turbidimetric method described in this paper is a convenient alternative for the routine quality control of daptomycin in its pharmaceutical dosage form.en
dc.description.affiliationSchool of Pharmaceutical Sciences, Universidade Estadual Paulista, Rod. Araraquara-Jaú, km 1, CEP 14801-902 Araraquara, SP, Brazil
dc.description.affiliationSchool of Pharmaceutical Sciences, Universidade Estadual Paulista, Rod. Araraquara-Jaú, km 1, CEP 14801-902 Araraquara, SP, Brazil
dc.description.affiliationUnespSchool of Pharmaceutical Sciences, Universidade Estadual Paulista, Rod. Araraquara-Jaú, km 1, CEP 14801-902 Araraquara, SP, Brazil
dc.description.affiliationUnespSchool of Pharmaceutical Sciences, Universidade Estadual Paulista, Rod. Araraquara-Jaú, km 1, CEP 14801-902 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação para o Desenvolvimento da Unesp (FUNDUNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent106-121
dc.identifierhttp://dx.doi.org/10.3390/pharmaceutics7030106
dc.identifier.citationPharmaceutics, v. 7, n. 3, p. 106-121, 2015.
dc.identifier.doi10.3390/pharmaceutics7030106
dc.identifier.filePMC4588188.pdf
dc.identifier.issn1999-4923
dc.identifier.pmcPMC4588188
dc.identifier.pubmed26184288
dc.identifier.urihttp://hdl.handle.net/11449/131470
dc.language.isoeng
dc.relation.ispartofPharmaceutics
dc.relation.ispartofjcr3.746
dc.relation.ispartofsjr0,949
dc.rights.accessRightsAcesso aberto
dc.sourcePubMed
dc.subjectAnalytical methoden
dc.subjectDaptomycinen
dc.subjectMicrobiological methoden
dc.subjectNew antimicrobial agenten
dc.subjectQuality controlen
dc.subjectTurbidimetric assayen
dc.titleRapid turbidimetric assay to determine the potency of daptomycin in lyophilized powderen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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