Bifunctional silica nanoparticles for the exploration of biofilms of Pseudomonas aeruginosa
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Hammer, Peter [UNESP]
Ribeiro, Sidney José Lima [UNESP]
Caiut, J. M A
Menu, M. J.
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Luminescent silica nanoparticles are frequently employed for biotechnology applications mainly because of their easy functionalization, photo-stability, and biocompatibility. Bifunctional silica nanoparticles (BSNPs) are described here as new efficient tools for investigating complex biological systems such as biofilms. Photoluminescence is brought about by the incorporation of a silylated ruthenium(II) complex. The surface properties of the silica particles were designed by reaction with amino-organosilanes, quaternary ammonium-organosilanes, carboxylate-organosilanes and hexamethyldisilazane. BSNPs were characterized extensively by DRIFT, 13C and 29Si solid state NMR, XPS, and photoluminescence. Zeta potential and contact angle measurements exhibited various surface properties (hydrophilic/hydrophobic balance and electric charge) according to the functional groups. Confocal laser scanning microscopy (CLSM) measurements showed that the spatial distribution of these nanoparticles inside a biofilm of Pseudomonas aeruginosa PAO1 depends more on their hydrophilic/hydrophobic characteristics than on their size. CLSM observations using two nanosized particles (25 and 68 nm) suggest that narrow diffusion paths exist through the extracellular polymeric substances matrix. © 2013 Copyright Taylor and Francis Group, LLC.
bifunctional silica nanoparticles, P. aeruginosa (PAO1) biofilms, photoluminescence, surface modification, biofilm, biotechnology, diffusion, functional group, luminescence, microbial community, microscopy, nanotechnology, particle size, polymer, reaction kinetics, ruthenium, silica, Pseudomonas aeruginosa
Biofouling, v. 29, n. 7, p. 775-788, 2013.