Serological and molecular diagnostic tests for canine visceral leishmaniasis in Brazilian endemic area: one out of five seronegative dogs are infected

dc.contributor.authorLopes, E. G.
dc.contributor.authorSeva, A. P.
dc.contributor.authorFerreira, F.
dc.contributor.authorNunes, C. M. [UNESP]
dc.contributor.authorKeid, L. B.
dc.contributor.authorHiramoto, R. M.
dc.contributor.authorFerreira, H. L.
dc.contributor.authorOliveira, T. M. F. S.
dc.contributor.authorBigotto, M. F. D.
dc.contributor.authorGalvis-Ovallos, F.
dc.contributor.authorGalati, E. A. B.
dc.contributor.authorSoares, R. M.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAdolfo Lutz Inst
dc.date.accessioned2018-11-26T15:45:41Z
dc.date.available2018-11-26T15:45:41Z
dc.date.issued2017-09-01
dc.description.abstractEuthanasia of infected dogs is one of the measures adopted in Brazil to control visceral leishmaniasis (VL) in endemic areas. To detect infected dogs, animals are screened with the rapid test DPP (R) Visceral Canine Leishmaniasis for detection of antibodies against K26/K39 fusion antigens of amastigotes (DPP). DPP-positives are confirmed with an immunoenzymatic assay probing soluble antigens of promastigotes (ELISA), while DPP-negatives are considered free of infection. Here, 975 dogs from an endemic region were surveyed by using DPP, ELISA and real-time PCR (qPCR) for the diagnosis of VL. When DPP-negative dogs were tested by qPCR applied in blood and lymph node aspirates, 174/887 (19.6%) were positive in at least one sample. In a second sampling using 115 cases, the DPP-negative dogs were tested by qPCR in blood, lymph node and conjunctival swab samples, and 36/79 (45.6%) were positive in at least one sample. Low-to-moderate pairwise agreement was observed between all possible pair of tests. In conclusion, the official diagnosis of VL in dogs in Brazilian endemic areas failed to accuse an expressive number of infected animals and the impact of the low accuracy of serological tests in the success of euthanasia-based measure for VL control need to be assessed.en
dc.description.affiliationUniv Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet, Dept Med Vet, Campus Aracatuba, Aracatuba, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Med Vet, Fac Zootecnia & Engn Alimentos, Pirassununga, SP, Brazil
dc.description.affiliationAdolfo Lutz Inst, Ctr Parasitol & Micol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Saude Publ, Dept Epidemiol, Sao Paulo, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet, Dept Med Vet, Campus Aracatuba, Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2011/21796-2
dc.description.sponsorshipIdFAPESP: 2011/14892-5
dc.format.extent2436-2444
dc.identifierhttp://dx.doi.org/10.1017/S0950268817001443
dc.identifier.citationEpidemiology And Infection. New York: Cambridge Univ Press, v. 145, n. 12, p. 2436-2444, 2017.
dc.identifier.doi10.1017/S0950268817001443
dc.identifier.fileWOS000414606100003.pdf
dc.identifier.issn0950-2688
dc.identifier.urihttp://hdl.handle.net/11449/159905
dc.identifier.wosWOS:000414606100003
dc.language.isoeng
dc.publisherCambridge Univ Press
dc.relation.ispartofEpidemiology And Infection
dc.relation.ispartofsjr1,128
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectDogs
dc.subjectPCR
dc.subjectserodiagnosis
dc.subjectvisceral leishmaniasis
dc.titleSerological and molecular diagnostic tests for canine visceral leishmaniasis in Brazilian endemic area: one out of five seronegative dogs are infecteden
dc.typeArtigo
dcterms.licensehttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dcterms.rightsHolderCambridge Univ Press
unesp.author.lattes1892359871207408[4]
unesp.author.orcid0000-0002-5463-3845[4]

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