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Crystallization and preliminary X-ray diffraction analysis of a myotoxic Lys49-PLA(2) from Bothrops jararacussu venom complexed with p-bromophenacyl bromide

dc.contributor.authorMarchi-Salvador, D. P.
dc.contributor.authorFernandes, CAH
dc.contributor.authorAmui, S. F.
dc.contributor.authorSoares, A. M.
dc.contributor.authorFontes, MRM
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:49:21Z
dc.date.available2014-05-20T13:49:21Z
dc.date.issued2006-06-01
dc.description.abstractFor the first time, a non-catalytic and myotoxic Lys49-PLA(2) (BthTX-I from Bothrops jararacussu venom) has been crystallized with BPB inhibitor. X-ray diffraction data were collected and electron-density calculations showed that the ligand is bound to the His48 residue. BthTX-I with His48 chemically modified by BPB shows strongly reduced myotoxic and cytotoxic activities. This suggests a biological correlation between the modification of His48, which is associated with catalytic activity of PLA(2)s, and other toxicological activities of Lys49-PLA(2)s.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUSP, FCFRP, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil
dc.format.extent600-603
dc.identifierhttp://dx.doi.org/10.1107/S174430910601801X
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 600-603, 2006.
dc.identifier.doi10.1107/S174430910601801X
dc.identifier.issn1744-3091
dc.identifier.urihttp://hdl.handle.net/11449/17582
dc.identifier.wosWOS:000238067600031
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray diffraction analysis of a myotoxic Lys49-PLA(2) from Bothrops jararacussu venom complexed with p-bromophenacyl bromideen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
dspace.entity.typePublication
unesp.author.orcid0000-0002-4634-6221[5]
unesp.author.orcid0000-0001-6515-6872[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentFísica e Biofísica - IBBpt

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