Publicação: Crystallization and preliminary X-ray diffraction analysis of a myotoxic Lys49-PLA(2) from Bothrops jararacussu venom complexed with p-bromophenacyl bromide
dc.contributor.author | Marchi-Salvador, D. P. | |
dc.contributor.author | Fernandes, CAH | |
dc.contributor.author | Amui, S. F. | |
dc.contributor.author | Soares, A. M. | |
dc.contributor.author | Fontes, MRM | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2014-05-20T13:49:21Z | |
dc.date.available | 2014-05-20T13:49:21Z | |
dc.date.issued | 2006-06-01 | |
dc.description.abstract | For the first time, a non-catalytic and myotoxic Lys49-PLA(2) (BthTX-I from Bothrops jararacussu venom) has been crystallized with BPB inhibitor. X-ray diffraction data were collected and electron-density calculations showed that the ligand is bound to the His48 residue. BthTX-I with His48 chemically modified by BPB shows strongly reduced myotoxic and cytotoxic activities. This suggests a biological correlation between the modification of His48, which is associated with catalytic activity of PLA(2)s, and other toxicological activities of Lys49-PLA(2)s. | en |
dc.description.affiliation | Univ Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil | |
dc.description.affiliation | USP, FCFRP, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, Brazil | |
dc.description.affiliationUnesp | Univ Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil | |
dc.format.extent | 600-603 | |
dc.identifier | http://dx.doi.org/10.1107/S174430910601801X | |
dc.identifier.citation | Acta Crystallographica Section F-structural Biology and Crystallization Communications. Oxford: Blackwell Publishing, v. 62, p. 600-603, 2006. | |
dc.identifier.doi | 10.1107/S174430910601801X | |
dc.identifier.issn | 1744-3091 | |
dc.identifier.uri | http://hdl.handle.net/11449/17582 | |
dc.identifier.wos | WOS:000238067600031 | |
dc.language.iso | eng | |
dc.publisher | Blackwell Publishing | |
dc.relation.ispartof | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.title | Crystallization and preliminary X-ray diffraction analysis of a myotoxic Lys49-PLA(2) from Bothrops jararacussu venom complexed with p-bromophenacyl bromide | en |
dc.type | Artigo | |
dcterms.license | http://olabout.wiley.com/WileyCDA/Section/id-406071.html | |
dcterms.rightsHolder | Blackwell Publishing | |
dspace.entity.type | Publication | |
unesp.author.orcid | 0000-0002-4634-6221[5] | |
unesp.author.orcid | 0000-0001-6515-6872[2] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatu | pt |
unesp.department | Física e Biofísica - IBB | pt |
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