Experimental pulmonary paracoccidioidomycosis in the Syrian hamster: morphology and correlation of lesions with the immune response.

dc.contributor.authorTani, E. M.
dc.contributor.authorFranco, M.
dc.contributor.authorPeraçoli, M. T.
dc.contributor.authorMontenegro, M. R.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T03:10:43Z
dc.date.available2014-05-27T03:10:43Z
dc.date.issued1987-10-01
dc.description.abstractA model for pulmonary paracoccidioidomycosis in the hamster is described. The disease was induced by intratracheal inoculation of 1.7 x 10(5) viable yeast forms of P. brasiliensis. Lung histopathology, dissemination lesions and humoral and cellular immune responses were investigated at intervals up to 24 weeks after infection. Humoral immunity was studied by immunodiffusion and complement fixation tests. Cell-mediated immunity was evaluated in vitro by the macrophage migration inhibition test in the presence of phytohaemagglutinin and P. brasiliensis soluble antigen, and in vivo by the paracoccidioidin test. Thirty out of 35 infected animals (85.7%) developed pulmonary paracoccidioidomycosis. Dissemination lesions were observed in regional lymph nodes (82.8%), liver (8.5%) and spleen (5.7%). Lung involvement was mainly around bronchi and vessels. Regional lymph nodes were severely involved from the fourth week on, acquiring a pseudotumoral aspect at later stages. Specific antibodies were detected from the fourth week on, with titres increasing progressively. The cellular immune response to phytohaemagglutinin was intact throughout the experiment and the response to P. brasiliensis antigen was already detectable by the second week and remained positive to the end of the experiment. The skin test became positive from the fourth week on. Inoculation by the intratracheal route represents a highly effective way of infecting hamsters with P. brasiliensis, with the induction of localized disease, good antibody production and intact cell immunity.en
dc.format.extent291-300
dc.identifierhttp://dx.doi.org/10.1080/02681218780000331
dc.identifier.citationJournal of Medical and Veterinary Mycology, v. 25, n. 5, p. 291-300, 1987.
dc.identifier.doi10.1080/02681218780000331
dc.identifier.issn0268-1218
dc.identifier.scopus2-s2.0-0023423320
dc.identifier.urihttp://hdl.handle.net/11449/63812
dc.language.isoeng
dc.relation.ispartofJournal of Medical and Veterinary Mycology
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectfungus antibody
dc.subjectanimal
dc.subjectcellular immunity
dc.subjectdisease model
dc.subjectgranuloma
dc.subjecthamster
dc.subjectimmunology
dc.subjectleukocyte migration inhibition test
dc.subjectlung mycosis
dc.subjectlymph node
dc.subjectmale
dc.subjectParacoccidioides
dc.subjectpathology
dc.subjectskin test
dc.subjectSouth American blastomycosis
dc.subjectAnimal
dc.subjectAntibodies, Fungal
dc.subjectCell Migration Inhibition
dc.subjectDisease Models, Animal
dc.subjectGranuloma
dc.subjectHamsters
dc.subjectImmunity, Cellular
dc.subjectLung Diseases, Fungal
dc.subjectLymph Nodes
dc.subjectMale
dc.subjectMesocricetus
dc.subjectParacoccidioidomycosis
dc.subjectSkin Tests
dc.subjectSupport, Non-U.S. Gov't
dc.titleExperimental pulmonary paracoccidioidomycosis in the Syrian hamster: morphology and correlation of lesions with the immune response.en
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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