Amino acid, antioxidant and ion profiles of carpolobia lutea leaf (Polygalaceae)

dc.contributor.authorNwidu, Lucky Legbosi
dc.contributor.authorCilli, Eduardo Maffud [UNESP]
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.institutionNiger Delta University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T05:37:13Z
dc.date.available2022-04-29T05:37:13Z
dc.date.issued2012-10-01
dc.description.abstractPurpose: To evaluate the amino acid, antioxidant and ionic profiles of Carpolobia lutea leaf (Polygalaceae) extract (CLL). Methods: The powdered leaf was macerated and subjected to gradient solvent extraction with nhexane, chloroform, ethyl acetate and ethanol for 72 h to obtain their respective fractions. Amino acid analysis was by cation-exchange chromatography using automated amino acid analyser. Antioxidant potential was obtained by spectrophotometric assay using 2, 2-Diphenyl-1-picrylhydrazyl DPPH while elemental and ionic analyses were carried out by atomic absorption spectrophotometry and potentiometric titration, respectively. Results: Proline, alanine, serine, valine, glycine, glutamate and lysine were found in the ethanol fraction while lysine, phenyl alanine, glycine and serine were present in the ethyl acetate fraction but not in the non-polar fractions, n-hexane and chloroform. The ethyl acetate fraction contained more lysine, phenyl alanine, glycine and serine the other leaf fractions. Minimal radical scavenging activity of all the fractions was recorded. The most abundant cations in the extract were potassium and phosphorus (2.16 ± 0.05 and 1.90 ± 0.06 mg/g, respectively) while the most abundant anion was phosphate with a concentration of 23.23 ± 4.61 mg/g for the aqueous leaf fraction Conclusion: The study shows that CLL fractions contain variety of amino acids which could promote wound healing, as well as major and minor elemental ions which, as essential body electrolytes, are required for various metabolic processes in the body. © Pharmacotherapy Group.en
dc.description.affiliationDepartment of Pharmacology and Toxicology Faculty of Pharmacy Niger Delta University, Wilberforce Island, Bayelsa State
dc.description.affiliationDepartment of Biochemistry Chemistry Institute São Paulo State University - UNESP, CP 355, CEP 14801-970, Araraquara, SP
dc.description.affiliationDepartment of Organic Chemistry Chemistry Institute São Paulo State University - UNESP, CP 355, CEP 14801-970, Araraquara, SP
dc.description.affiliationUnespDepartment of Biochemistry Chemistry Institute São Paulo State University - UNESP, CP 355, CEP 14801-970, Araraquara, SP
dc.description.affiliationUnespDepartment of Organic Chemistry Chemistry Institute São Paulo State University - UNESP, CP 355, CEP 14801-970, Araraquara, SP
dc.format.extent807-813
dc.identifier.citationTropical Journal of Pharmaceutical Research, v. 11, n. 5, p. 807-813, 2012.
dc.identifier.issn1596-5996
dc.identifier.issn1596-9827
dc.identifier.scopus2-s2.0-84868021509
dc.identifier.urihttp://hdl.handle.net/11449/227016
dc.language.isoeng
dc.relation.ispartofTropical Journal of Pharmaceutical Research
dc.sourceScopus
dc.subjectAmino acid
dc.subjectAntioxidant
dc.subjectCarpolobia leaf
dc.subjectIonic profile
dc.subjectPolygalaceae
dc.titleAmino acid, antioxidant and ion profiles of carpolobia lutea leaf (Polygalaceae)en
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.departmentBioquímica e Tecnologia - IQARpt
unesp.departmentQuímica Orgânica - IQARpt

Arquivos