Evaluation of photosensitizer-containing superhydrophobic surfaces for the antibacterial treatment of periodontal biofilms

dc.contributor.authorTonon, Caroline Coradi
dc.contributor.authorAshraf, Shoaib
dc.contributor.authorde Souza Rastelli, Alessandra Nara [UNESP]
dc.contributor.authorGhosh, Goutam
dc.contributor.authorHasan, Tayyaba
dc.contributor.authorXu, QianFeng
dc.contributor.authorGreer, Alexander
dc.contributor.authorLyons, Alan M.
dc.contributor.institutionMassachusetts General Hospital and Harvard Medical School
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionHarvard University and Massachusetts Institute of Technology
dc.contributor.institutionSingletO2 Therapeutics LLC
dc.contributor.institutionCity University of New York
dc.contributor.institutionThe Graduate Center of the City University of New York
dc.date.accessioned2023-03-01T20:48:17Z
dc.date.available2023-03-01T20:48:17Z
dc.date.issued2022-08-01
dc.description.abstractAntimicrobial photodynamic therapy (aPDT) is a promising approach to control biofilms involved in periodontal diseases. However, certain challenges, such as staining of teeth, preferential interaction of photosensitizer (PS) with Gram-positive versus Gram-negative bacteria, and insufficient oxygen in hypoxic periodontal pockets have presented barriers to its use in the clinic. To overcome these challenges, a novel superhydrophobic (SH) film that generates airborne singlet oxygen has been developed. The SH-aPDT approach isolates the PS onto a topologically rough solid SH film on which channels allow air to diffuse to the PS surface, thus ensuring sufficient oxygen supply. Upon illumination, gas phase singlet oxygen (1O2) is produced and diffuses from the SH surface to the underlying biofilm. The killing efficacy was assessed as a function of transmitted fluence (17.9–89.5 J/cm2) and chorin e6 loading (96–1110 nmol/cm2) by counting of colony forming units, biofilm metabolism by XTT and confocal microscopy. The decrease in viability of both Gram-positive and Gram-negative bacteria in a multi-species biofilm was found to be linearly dependent on the fluence as well as the loading of the PS up to 71.6 J/cm2 when 1110 nmols/cm2 of chlorin e6 was used. A > 4.6 log bacterial reduction was observed under these conditions (p < 0.05). This novel SH-aPDT approach shows promise as an effective method to disinfect multi-species bacterial biofilms associated with periodontal disease and will be evaluated in animal models in future studies.en
dc.description.affiliationWellman Center for Photomedicine Massachusetts General Hospital and Harvard Medical School, 40 Blossom St
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry Araraquara São Paulo State University-UNESP, 1680 Humaitá St.
dc.description.affiliationDivision of Health Sciences and Technology Harvard University and Massachusetts Institute of Technology
dc.description.affiliationSingletO2 Therapeutics LLC, TechBox, Suite 3, 75 Clinton St
dc.description.affiliationDepartment of Chemistry College of Staten Island City University of New York, Staten Island
dc.description.affiliationPh.D. Program in Chemistry The Graduate Center of the City University of New York, 365 Fifth Avenue
dc.description.affiliationDepartment of Chemistry Brooklyn College City University of New York
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry Araraquara São Paulo State University-UNESP, 1680 Humaitá St.
dc.description.sponsorshipNational Institute of Dental and Craniofacial Research
dc.description.sponsorshipIdNational Institute of Dental and Craniofacial Research: 2R44DE026083-03
dc.identifierhttp://dx.doi.org/10.1016/j.jphotobiol.2022.112458
dc.identifier.citationJournal of Photochemistry and Photobiology B: Biology, v. 233.
dc.identifier.doi10.1016/j.jphotobiol.2022.112458
dc.identifier.issn1873-2682
dc.identifier.issn1011-1344
dc.identifier.scopus2-s2.0-85131551839
dc.identifier.urihttp://hdl.handle.net/11449/241128
dc.language.isoeng
dc.relation.ispartofJournal of Photochemistry and Photobiology B: Biology
dc.sourceScopus
dc.subjectAntimicrobial photodynamic inactivation
dc.subjectBacterial biofilms
dc.subjectChlorin e6
dc.subjectPeriodontal disease
dc.subjectSinglet oxygen
dc.subjectSuperhydrophobicity
dc.titleEvaluation of photosensitizer-containing superhydrophobic surfaces for the antibacterial treatment of periodontal biofilmsen
dc.typeArtigo

Arquivos