A metabolic pathway assembled by enzyme selection may support herbivory of leaf-cutter ants on plant starch

dc.contributor.authorBacci, Maurício [UNESP]
dc.contributor.authorBueno, Odair Correa [UNESP]
dc.contributor.authorRodrigues, André [UNESP]
dc.contributor.authorPagnocca, Fernando Carlos [UNESP]
dc.contributor.authorSomera, Alexandre Favarin [UNESP]
dc.contributor.authorSilva, Aline
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Santa Cruz (UESC)
dc.date.accessioned2014-05-27T11:29:01Z
dc.date.available2014-05-27T11:29:01Z
dc.date.issued2013-05-01
dc.description.abstractMutualistic associations shape the evolution in different organism groups. The association between the leaf-cutter ant Atta sexdens and the basidiomycete fungus Leucoagaricus gongylophorus has enabled them to degrade starch from plant material generating glucose, which is a major food source for both mutualists. Starch degradation is promoted by enzymes contained in the fecal fluid that ants deposit on the fungus culture in cut leaves inside the nests. To understand the dynamics of starch degradation in ant nests, we purified and characterized starch degrading enzymes from the ant fecal fluid and from laboratory cultures of L. gongylophorus and found that the ants intestine positively selects fungal α-amylase and a maltase likely produced by the ants, as a negative selection is imposed to fungal maltase and ant α-amylases. Selected enzymes are more resistant to catabolic repression by glucose and proposed to structure a metabolic pathway in which the fungal α-amylase initiates starch catalysis to generate byproducts which are sequentially degraded by the maltase to produce glucose. The pathway is responsible for effective degradation of starch and proposed to represent a major evolutionary innovation enabling efficient starch assimilation from plant material by leaf-cutters. © 2013 Elsevier Ltd.en
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências de Rio Claro Centro de Estudos de Insetos Sociais/Departamento de Bioquímica e Microbiologia
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências de Rio Claro Centro de Estudos de Insetos Sociais/Departamento de Biologia
dc.description.affiliationDepartamento de Ciências Biológicas Universidade Estadual de Santa Cruz
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências de Rio Claro Centro de Estudos de Insetos Sociais/Departamento de Bioquímica e Microbiologia
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências de Rio Claro Centro de Estudos de Insetos Sociais/Departamento de Biologia
dc.format.extent525-531
dc.identifierhttp://dx.doi.org/10.1016/j.jinsphys.2013.02.007
dc.identifier.citationJournal of Insect Physiology, v. 59, n. 5, p. 525-531, 2013.
dc.identifier.doi10.1016/j.jinsphys.2013.02.007
dc.identifier.issn0022-1910
dc.identifier.lattes1050709055776428
dc.identifier.lattes8302605179522059
dc.identifier.orcid0000-0002-4164-9362
dc.identifier.scopus2-s2.0-84876337896
dc.identifier.urihttp://hdl.handle.net/11449/75224
dc.identifier.wosWOS:000319087700001
dc.language.isoeng
dc.relation.ispartofJournal of Insect Physiology
dc.relation.ispartofjcr2.733
dc.relation.ispartofsjr1,265
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAmylase
dc.subjectAttini
dc.subjectMaltase
dc.subjectMutualism
dc.subjectant
dc.subjectbiodegradation
dc.subjectentomology
dc.subjectenzyme activity
dc.subjectevolutionary biology
dc.subjectfungus
dc.subjectglucose
dc.subjectherbivory
dc.subjectmetabolism
dc.subjectmutualism
dc.subjectstarch
dc.titleA metabolic pathway assembled by enzyme selection may support herbivory of leaf-cutter ants on plant starchen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes1050709055776428
unesp.author.lattes8302605179522059[4]
unesp.author.lattes8538509657578022[3]
unesp.author.orcid0000-0002-4164-9362[3]
unesp.author.orcid0000-0002-5619-1411[1]
unesp.author.orcid0000-0002-5026-1933[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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