Vascular expression driven by the promoter of a gene encoding a high-affinity potassium transporter HAK5 from Eucalyptus grandis
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2017-11-01
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Costa, Carolina S. [UNESP]
Bravo, Juliana P. [UNESP]
Ribeiro, Cíntia L. [UNESP]
Soprano, Adriana S. [UNESP]
Sassaki, Flávio T. [UNESP]
Maia, Ivan G. [UNESP]
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The construction of expression cassettes harboring tissue-specific promoters is a viable alternative to limit transgene expression to specific organs and cell types. In this study, we have functionally characterized the promoter of a Eucalyptus grandis gene encoding a putative high-affinity HAK5-like potassium (K+) transporter (designated EgHAK5) showing root-specific expression. The ability of the EgHAK 5′-flanking region (~1.3 kb) to drive root-specific expression of a reporter gene (β-glucuronidase; GUS) was examined using transgenic tobacco plants. Histochemical analysis revealed enhanced GUS staining in the vasculature of leaves, hypocotyls and roots, which was also confirmed in histological cross-sections. Moreover, the relative expression of GUS in the roots of the generated transgenic lines was increased in response to K+ starvation. Overall, our results indicate that, in a heterologous system, the EgHAK5 promoter shows expression in vascular tissues, mainly within the phloem, and is up-regulated upon potassium deprivation.
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Eucalyptus, Potassium transporter, Promoter, Root, Vascular expression
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Plant Cell, Tissue and Organ Culture, v. 131, n. 2, p. 213-222, 2017.