Infecção por Leishmania sp. em cães de Florianópolis, Santa Catarina, Brasil

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Pacheco, Acácio Duarte [UNESP]
Laurenti, Marcia Dalastra
de Lima, Valéria Marçal Félix [UNESP]
Tomokame, Thaise Yumie
Marcondes, Mary [UNESP]

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The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florianópolis.



Diagnosis, ELISA, IFAT, PCR, Visceral leishmaniasis

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Brazilian Journal of Veterinary Research and Animal Science, v. 50, n. 3, p. 220-225, 2013.